Influenza A H4N6 HA / Hemagglutinin Antibody

Influenza A H4N6 Hemagglutinin / HA Antibody

H4N6 Hemagglutinin / HA Antibody Product Information

• Immunogen: Recombinant H4N6 HA protein ( Catalog#11714-V08H )
• Antibody Type: Mouse Monoclonal Antibody ( Mouse mAb Service Platform )
• Clone ID: 3E10B1
• Ig Type: Mouse IgG1
• Formulation: 0.2 μm filtered solution in PBS with 5% trehalose
• Preparation: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, human cell-derived, recombinant Influenza A virus H4N6 Hemagglutinin. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography

H4N6 Hemagglutinin / HA Antibody Usage Guide

• Specificity: H4N6 (A/mallard/Ohio/657/2002) HA
• Has cross-reactivity in ELISA with

  H2N2 (A/Canada/720/2005) HA

  No cross-reactivity in ELISA with

  H1N1 (A/California/07/2009) HA

  H3N2 (A/Brisbane/10/2007) HA

  H5N1 (A/Anhui/1/2005) HA

  H4N6 (A/Swine/Ontario/01911-1/99) HA

  H6N1 (A/northern shoveler/California/HKWF115/2007) HA

  H7N7 (A/chicken/Netherlands/1/03) HA

  H8N4 (A/pintail duck/Alberta/114/1979) HA

  H9N2 (A/Hong Kong/1073/99) HA

  H10N3 (A/duck/Hong Kong/786/1979) HA

  H11N2 (A/duck/Yangzhou/906/2002) HA

  H12N5 (A/green-winged teal/ALB/199/1991) HA

  H13N8 (A/black-headed gull/Netherlands/1/00) HA

  H16N3 (A/black-headed gull/Sweden/5/99) HA

  Influenza B (B/Florida/4/2006) HA

• Western blot: This antibody can be used at 1 - 2 μg/mL with the appropriate secondary reagents to detect H4N6 HA in WB. Using a DAB detection system, the detection limit for H4N6 HA is approximately 4 ng/lane under non-reducing conditions and 20 ng/lane under reducing conditions
• Direct ELISA: This antibody can be used at 0.5 - 1 μg/mL with the appropriate secondary reagents to detect H4N6 HA. The detection limit for H4N6 HA is approximately 0.039 ng/well
• Storage: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
  Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

Related Influenza Virus Research Tools

• Influenza Virus Research Tools
• Influenza Hemagglutinin & Antibody
• Influenza Hemagglutinin Proteins
• H4N6 Hemagglutinin & Antibody
• A/mallard/Ohio/657/2002

H4N6 Hemagglutinin / HA Antibody Background

Influenza (flu) is a respiratory infection in mammals and birds. This virus is divided into three main types (A, B and C). Influenza A is found in a wide variety of bird and mammal species and is further divided into subtypes based on differences in the membrane proteins hemagglutinin (HA) and neuraminidase (NA).

H4N6 is a subtype of Influenza A. Hemagglutinin (HA) is a single-pass type I  integral membrane glycoprotein from the influenza virus, and comprises over 80% of the envelope proteins present in the virus particle. The HA is a trimer with a receptor binding pocket on the globular head of each monomer. In natural infection, inactive HA is matured into HA1 and HA2 outside the cell by one or more trypsin-like, arginine-specific endoprotease secreted by the bronchial epithelial cells. Binding of HA to sialic acid-containing receptors on the surface of its target cell brings about the attachment of the virus particle to the cell and forms a endosome. Low pH in endosomes induce an irreversible conformational change in HA2, releasing the hydrophobic portion “fusion peptide”. After which, virus penetrates the cell and pours its contents including the RNA genome into the cytoplasm mediated by fusion of the endocytosed virus particle’s own membrane and the endosomal membrane. Hemagglutinin plays a major role in the determination of host range restriction and virulence.

References

1. Barman, S. et al., 2000, J. Virol. 74: 6538-45.
2. Suzuki, T. et al., 2005, J. Virol. 79: 11705-15.
3. Shinya K, et al., 2006, Nature. 440 (7083): 435-6.
4. Marjuki, H. et al., 2006, J. Biol. Chem. 281: 16707-15.
5. von, Itzstein, M. 2007, Nat. Rev. Drug. Discov. 6: 967-74.
6. Christophe F, et al., 2009, Science. 324:1557-61.