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Influenza A H4N6 (A/mallard/Ohio/657/2002) Hemagglutinin / HA ELISA Pair Set

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Materials provided
Capture Ab:0.5 mg/mL of mouse anti-H4N6 (A/mallard/Ohio/657/2002) HA monoclonal antibody, Dilute to a working concentration of 2 μg/mL in CBS before coating.
Detection Ab:0.4 mg/mL mouse anti-H4N6 (A/mallard/Ohio/657/2002) HA monoclonal antibody conjugated to horseradish-peroxidase (HRP). Dilute to working concentration of 1 μg/mL in detection antibody dilution buffer before use.
Standard:Each vial contains 140 ng of recombinant H4N6 (A/mallard/Ohio/657/2002) HA. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20℃ to -70℃ in a manual defrost freezer. A seven-point standard curve usi ng 2-fold serial dilutions in sample dilution buffer, and a high standard of 7 ng/mL is recommended.
Sensitivity
The minimum detectable dose of H4N6 (A/mallard/Ohio/657/2002) HA was determined to be approximately 109.38 pg/ml. This is defined as at least three times standard deviations above the mean optical density of 10 replicates of the zero standard.
Principle of the product
The H4N6 (A/mallard/Ohio/657/2002) HA ELISA Pair Set is for the quantitative determination of H4N6 (A/mallard/Ohio/657/2002) HA.
This ELISA Pair Set contains the basic components required for the development of sandwich ELISAs.
The Sino Biological ELISA Pair Set is a solid phase sandwich ELISA (Enzyme-Linked Immunosorbent Assay). It utilizes a monoclonal antibody specific for H4N6 (A/mallard/Ohio/657/2002) HA coated on a 96-well plate. Standards and samples are added to the wells, and any H4N6 (A/mallard/Ohio/657/2002) HA present binds to the immobilized antibody. The wells are washed and a horseradish peroxidase conjugated mouse anti-H4N6 (A/mallard/Ohio/657/2002) HA monoclonal antibody is then added, producing an antibody-antigen-antibody “sandwich”. The wells are again washed and TMB substrate solution is loaded, which produces color in proportion to the amount of H4N6 (A/mallard/Ohio/657/2002) HA present in the sample. To end the enzyme reaction, the stop solution is added and absorbances of the microwell are read at 450 nm.
Storage
Capture Antibody: Aliquot and store at -20℃ to -70℃ for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Detection Antibody: Protect it from prolonged exposure to light. Aliquot and store at -20℃ to -70℃ and for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Standard: Store lyophilized Standard at -20℃ to -70℃ for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -20℃ to -70℃ for up to 1 month. Avoid repeated freeze-thaw cycles.
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  • Influenza A H4N6 (A/mallard/Ohio/657/2002) Hemagglutinin / HA ELISA standard curve
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