H3N2 (A/Perth/16/2009) HA1 Protein (His Tag)

Hemagglutinin subunit 1 (Influenza A virus (A/Perth/16/2009(H3N2)) Protein Datasheet

H3N2 (A/Perth/16/2009) HA1 Protein Product Information

• Synonym: HA1
• Protein Construction: A DNA sequence encoding the N-terminal segment (Met 1-Arg 345) of the influenza hemagglutin (A/Perth/16/2009(H3N2)) ( ACS71642.1)， termed as HA1, was fused with a polyhistidine tag at the C-terminus.
• Source: Influenza A Virus
• Expression Host: Human Cells

H3N2 (A/Perth/16/2009) HA1 Protein QC Testing

• Purity: > 95 % as determined by SDS-PAGE.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Gln 17
• Molecular Mass: The secreted recombinant HA1 subunit of influenza A H3N2 HA (A/Perth/16/2009(H3N2)) comprises 340 amino acids and has a predicted molecular mass of 38 kDa. As a result of glycosylation, it migrates as an approximately 60 kDa band in reduced SDS-PAGE.
• Formulation: Lyophilized from sterile PBS , pH 7.4
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: H3N2(A/Perth/16/2009)HA1 protein

H3N2 (A/Perth/16/2009) HA1 Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

H3N2 (A/Perth/16/2009) HA1 Protein Description

Influenza (flu) is a respiratory infection in mammals and birds. This virus is divided into three main types (A, B and C). Influenza A is found in a wide variety of bird and mammal species and is further divided into subtypes based on differences in the membrane proteins hemagglutinin (HA) and neuraminidase (NA). H3N2 is a subtype of Influenza A. Hemagglutinin (HA) is a single-pass type I integral membrane glycoprotein from the influenza virus, and comprises over 80% of the envelope proteins present in the virus particle. The HA is a trimer with a receptor binding pocket on the globular head of each monomer. In natural infection, inactive HA is matured into HA1 and HA2 outside the cell by one or more trypsin-like, arginine-specific endoprotease secreted by the bronchial epithelial cells. Binding of HA to sialic acid-containing receptors on the surface of its target cell brings about the attachment of the virus particle to the cell and forms a endosome. Low pH in endosomes induce an irreversible conformational change in HA2, releasing the hydrophobic portion "fusion peptide". After which, virus penetrates the cell and pours its contents including the RNA genome into the cytoplasm mediated by fusion of the endocytosed virus particle's own membrane and the endosomal membrane. Hemagglutinin plays a major role in the determination of host range restriction and virulence.

References

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