Human H2BFWT Gene cDNA Clone / ORF Clone

Catalog	Vector (Price)	Quantity	In Stock	Operation
HG13599-G	Cloning vector ($95) pCMV / hygro ($295) pCMV / hygro - HA ($295) pCMV / hygro - His ($295) pCMV / hygro - Myc ($295) pCMV / hygro - FLAG ($295)		YES
* pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors. Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

H2BFWT cDNA Clone Datasheet

H2BFWT cDNA Clone Product Information

Gene Bank Ref.ID:	NM_001002916.3
cDNA Size:	528
Gene Synonym:	H2BFWT
Source:	Human

H2BFWT cDNA Clone Description

cDNA Description:	ORF Clone of Homo sapiens H2B histone family, member W, testis-specific DNA.
Vector:	Cloning Vector: pGEM-T Vector Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG
Sequence Description:	Identical with the Gene Bank Ref. ID sequence except for the point mutation 368 G/A resulting in the amino acid Arg substitution by His.

H2BFWT cDNA Clone Shipping

5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

H2BFWT cDNA Clone Usage Protocol

Cut along the circle edge of the spot (arrow points to) to get the filter paper.
Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
Transfer the tube to incubation at 95℃ for 15-30 min.
At the end of the incubation pulse vortex, or gently tap the tube.
Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

H2BFWT cDNA Clone Sequence and Quality Control

Sequence:

1        ATGCTGCGTA CCGAAGTGCC CCGGCTTCCC CGGTCCACAA CCGCCATTGT CTGGTCGTGC
61       CATCTAATGG CCACTGCCTC CGCCATGGCT GGACCTTCCT CTGAGACGAC CTCTGAGGAA
121      CAGCTGATCA CCCAGGAGCC CAAAGAGGCC AACTCCACTA CGTCCCAGAA GCAGAGCAAG
181      CAGAGGAAGC GAGGGCGCCA TGGGCCCCGC AGGTGCCACT CCAACTGCCG CGGGGACAGC
241      TTCGCCACCT ATTTCCGCCG GGTGCTGAAG CAGGTTCACC AGGGCCTCAG CCTTTCCCGG
301      GAGGCCGTGA GTGTCATGGA TTCTTTGGTT CATGACATAT TGGACCGCAT CGCCACCGAG
361      GCTGGTCACC TGGCCCGCTC CACCAAGCGC CAGACCATCA CTGCCTGGGA GACCCGGATG
421      GCTGTGCGCC TGCTGCTGCC GGGGCAGATG GGCAAGCTCG CCGAGTCCGA AGGCACGAAG
481      GCTGTCCTCA GAACTTCACT GTATGCCATA CAGCAACAGA GAAAGTGA

Quality Control:cDNA sequencing results is available upon request.