Influenza A H1N3 (A/duck/NZL/160/1976) Hemagglutinin Gene cDNA Clone / ORF Clone (codon optimized)

Influenza A H1N3 (A/duck/NZL/160/1976) cDNA Clone Datasheet

Influenza A H1N3 (A/duck/NZL/160/1976) cDNA Clone Product Information

• cDNA Size: 1723
• Gene Synonym: HA1, Hemagglutinin
• Source: Influenza Virus

Influenza A H1N3 (A/duck/NZL/160/1976) cDNA Clone Description

• cDNA Description: ORF Clone of Influenza A H1N3 (A/duck/NZL/160/1976) HA DNA.
• Vector: Vector 3. Restriction site: HindIII+XbaI

Influenza A H1N3 (A/duck/NZL/160/1976) cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

Influenza A H1N3 (A/duck/NZL/160/1976) cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95℃ for 15-30 min.
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

Influenza A H1N3 (A/duck/NZL/160/1976) cDNA Clone Sequence and Quality Control

Sequence:

1        AAGCTTGCCG CCACCATGGA GGCAAGACTG TTTGTGCTGT TCTGTACCTT CACAGTGCTG
61       AAAGCAGACA TCATCTGTGT GGGCTACCAT GCCAACAACA GCACAGACAC AGTGGACACA
121      GTGTTGGAGA AGAATGTGAC AGTGACCCAC TCTGTGAACC TGTTGGAGTC CAGCCACAAT
181      GGCAAACTGT GTTCCCTGAA TGGCATCCCT CCACTCCAAC TTGGCAAGTG TAATGTGGCT
241      GGCTGGCTGC TGGGCAACCC TGAGTGTGAC CAACTTCTGA CAGCCAACTC CTGGTCCTAC
301      ATCATTGAGA CCAGCAACTC TGAGAATGGC ACTTGTTACC CTGGAGAGTT CATTGACTAT
361      GAGGAACTGA GGGAACAACT TTCCTCTGTG TCCTCCTTTG AGAAGTTTGA GATTTTTCCA
421      AAGGCATCCT CCTGGCCAAA CCATGAGACC ACCAAGGGAG TGACAGCAGC CTGTCCATAC
481      CTGGGCAACA GCAACTTCTA CAGGAACCTG CTGTGGATTA CCAAGAAGGG CAACTCCTAC
541      CCTGAACTGA GCAAGTCCTA CACCAACAAC AAGGGCAAGG AGGTGCTGAT TCTGTGGGGA
601      GTGCATCACC CTCCAAGCAC CAATGAACAA CAGAACCTCT ACCAGAATGC CAATGCCTAT
661      GTGTCTGTGG GCTCCAGCAA ATACAACAGG AGGTTCACAC CTGAGATTGC TGCCAGACCA
721      AAGGTGAGGG GACAAGCAGG CAGGATGAAC TACTACTGGA CCCTGCTGGA CCAGGGAGAC
781      ACCATCACCT TTGAGGCTAC AGGCAACCTG ATTGCCCCAT GGTATGCCTT TGCCCTGAAC
841      AAGAACTCTG ACTCTGGCAT AATGATGAGT GATGCCCCTG TGCATAACTG TGACACCAAG
901      TGTCAGACAC CTCATGGAGC CCTGAACTCC TCCCTGCCAT TCCAGAATGT GCATCCAATC
961      ACCATTGGAG AGTGTCCAAA ATACATCAAG AGCACCAAAC TGAGGATGGC TACAGGACTG
1021     AGGAATGTGC CAAGCATCCA GAGCAGGGGA CTGTTTGGAG CCATTGCTGG CTTCATTGAG
1081     GGAGGCTGGA CAGGGATGAT TGATGGCTGG TATGGCTACC ACCACCAGAA TGAACAGGGC
1141     TCTGGCTATG CTGCTGACCA GAAGAGCACC CAGAATGCCA TTGATGGCAT CACCAACAAG
1201     GTGAACTCTG TGATTGAGAA GATGAACACC CAGTTCACAG CAGTGGGCAA GGAGTTCAAC
1261     AACTTGGAGA AGAGGATTGA GAACCTGAAC AAGAAGGTGG ATGATGGCTT CCTGGATGTG
1321     TGGACCTACA ATGCTGAACT GCTGGTGCTG TTGGAGAATG AGAGGACCCT GGACTTCCAT
1381     GACAGCAATG TGAGGAACCT CTATGAGAAG GTGAAGAGCC AACTTAGGAA CAATGCCAAG
1441     GAGATTGGCA ATGGCTGTTT TGAGTTCTAC CACAAGTGTG ATGATGAGTG TATGGAGTCT
1501     GTGAAGAATG GCACCTATGA CTACCCAAAA TACTCTGAGG AGAGCAAACT GAACAGGGAG
1561     GAGATTGATG GAGTGAAATT GGAGAGTATG GGAGTCTACC AGATTCTGGC TATCTACAGC
1621     ACAGTGGCAT CCTCCCTGGT GCTGCTGGTG TCCCTGGGAG CCATCTCCTT CTGGATGTGT
1681     AGCAATGGCT CCCTCCAATG TAGGGTGTGT ATTTAAATCT AGA

 

Quality Control:cDNA sequencing results is available upon request.

Please use the cDNA nucleotide sequence above for sequence blast instead of copying/pasting the sequence from COA (pdf).