Influenza A H1N3 HA / Hemagglutinin Antibody

H1N3 Hemagglutinin / HA Antibody Datasheet

H1N3 Hemagglutinin / HA Antibody Product Information

• Immunogen: Recombinant H1N3 HA protein (Catalog #11685-V08H)
• Antibody Type: Rabbit Polyclonal Antibody ( Antibody Purification Platform )
• Ig Type: Rabbit IgG
• Formulation: 0.2 μm filtered solution in PBS with 5% trehalose
• Preparation: Produced in rabbits immunized with purified, human cell-derived, recombinant Influenza A virus H1N3 Hemagglutinin (rV Influenza A virus H1N3 Hemagglutinin; Catalog #11685-V08H; Met 1- Gln 529; ABB20429.1). Total IgG was purified by Protein A affinity chromatography.

H1N3 Hemagglutinin / HA Antibody Usage Guide

• Specificity: Influenza A virus H1N3 (A/duck/NZL/160/1976) Hemagglutinin
• Western blot: This antibody can be used at 1-2 μg/mL with the appropriate secondary reagents to detect H1N3 HA in WB.
• Direct ELISA: This antibody can be used at 0.5-1 μg/mL with the appropriate secondary reagents to detect H1N3 HA. The detection limit for H1N3 HA is approximately 0.00245 ng/well.
• Storage: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
  Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

Related Influenza Virus Research Tools

• Influenza Virus Research Tools
• Influenza Hemagglutinin & Antibody
• Influenza Hemagglutinin Proteins
• H1N3 Hemagglutinin & Antibody
• A/duck/NZL/160/1976 (A/duck/NZL/160/76)

H1N3 Hemagglutinin / HA Antibody Background

Influenza (flu) is a respiratory infection in mammals and birds. This virus is divided into three main types (A, B and C). Influenza A is found in a wide variety of bird and mammal species and is further divided into subtypes based on differences in the membrane proteins hemagglutinin (HA) and neuraminidase (NA). Hemagglutinin (HA) is a single-pass type I  integral membrane glycoprotein from the influenza virus, and comprises over 80% of the envelope proteins present in the virus particle. The HA is a trimer with a receptor binding pocket on the globular head of each monomer. In natural infection, inactive HA is matured into HA1 and HA2 outside the cell by one or more trypsin-like, arginine-specific endoprotease secreted by the bronchial epithelial cells. Binding of HA to sialic acid-containing receptors on the surface of its target cell brings about the attachment of the virus particle to the cell and forms a endosome. Low pH in endosomes induce an irreversible conformational change in HA2, releasing the hydrophobic portion “fusion peptide”. After which, virus penetrates the cell and pours its contents including the RNA genome into the cytoplasm mediated by fusion of the endocytosed virus particle’s own membrane and the endosomal membrane. Hemagglutinin plays a major role in the determination of host range restriction and virulence.

References

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