Influenza A H1N2 (A/swine/Guangxi/13/2006) Hemagglutinin Gene cDNA Clone / ORF Clone (codon optimized)

Influenza A H1N2 (A/swine/Guangxi/13/2006) cDNA Clone Datasheet

Influenza A H1N2 (A/swine/Guangxi/13/2006) cDNA Clone Product Information

• cDNA Size: 1723
• Gene Synonym: HA1, Hemagglutinin
• Source: Influenza Virus

Influenza A H1N2 (A/swine/Guangxi/13/2006) cDNA Clone Description

• cDNA Description: ORF Clone of Influenza A H1N2 (A/swine/Guangxi/13/2006) HA DNA.

  This cDNA clone has gone through customized codon optimization in order to obtain high level of protein expression in particular cell lines. Therefore, although the translated amino acid sequence is identical to the amino sequence on Gene Bank, the DNA sequence is different from that on Gene Bank.

• Vector: Vector 3. Restriction site: KpnI + XbaI

Influenza A H1N2 (A/swine/Guangxi/13/2006) cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

Influenza A H1N2 (A/swine/Guangxi/13/2006) cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O . Ensure the filter paper is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95℃ for 15-30 min.
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the scattered filter paper from the elute. The elute now contains the purified DNA.

Influenza A H1N2 (A/swine/Guangxi/13/2006) cDNA Clone Sequence and Quality Control

Sequence:

1        AAGCTTGCCG CCACCATGAA GGCTATCCTG GTGGTGCTGC TCTACACCTT CACCACAGCC
61       TCTGCTGACA CCCTGTGTAT TGGCTACCAT GCCAACAACA GCACAGACAC AGTGGACACA
121      GTGTTGGAGA AGAATGTGAC AGTGACCCAC TCTGTGAACC TGTTGGAGGA CAGACACAAT
181      GGCAAACTGT GTAAACTGAG GGGAGTGGCT CCACTGCACC TGGGCAAGTG TAACATTGCT
241      GGCTGGCTGC TGGGCAACCC TGAGTGTGAG TCCCTGTTCA CAGCCTCCTC CTGGTCCTAC
301      ATTGTGGAGA CCAGCAACTC TGACAATGGC ACTTGTTACC CTGGAGACTT CATTGACTAT
361      GAGGAACTGA GGGAACAACT TTCCTCTGTG TCCTCCTTTG AGAGGTTTGA GATTTTTCCA
421      AAGACCTCCT CCTGGCCTGA CCATGACAGC AACAGGGGAG TGACAGCAGC CTGTCCACAT
481      GCTGGAGCCA ACTCCTTCTA CAGGAACCTG ATTTGGCTGG TGAAGAAGGG CAACTCCTAC
541      CCAAAACTGA GCAAGTCCTA CATCAACAAC AAGGGCAAGG AGGTGCTGGT GCTGTGGGGC
601      ATCCACCACC CTCCAACCTC TGCCAACCAA CAGTCCCTCT ACCAGAATGC TGATGCCTAT
661      GTGTTTGTGG GCTCCAGCAG ATACAGCAAG AAGTTCAAGC CTGAGATTGC CACCAGACCA
721      AAGGTGAGGG ACCAGGCTGG CAGGATGAAC TACTACTGGA CCCTGGTGGA ACCTGGAGAC
781      AAGATTACCT TTGAGGCTAC AGGCAACCTG GTGGTGCCAA GATATGCCTT TGCTATGAAG
841      AGGAACTCTG GCTCTGGCAT CATCATCTCT GACACCTCTG TCCATGACTG TAACACCACT
901      TGTCAGACAC CAAAGGGAGC CATCAACACC TCCCTGCCAT TCCAGAACAT CCACCCTGTG
961      ACCATTGGAG AGTGTCCAAA ATATGTGAAG AGCACCAAAC TGAGGATGGC TACAGGACTG
1021     AGGAATGTGC CAAGCATCCA GAGCAGGGGA CTGTTTGGAG CCATTGCTGG CTTCATTGAG
1081     GGAGGCTGGA CAGGGATGAT TGATGGCTGG TATGGCTACC ACCACCAGAA TGAACAGGGC
1141     TCTGGCTATG CTGCTGACCT GAAAAGCACC CAGAATGCCA TTGATGGCAT CACCAACAAG
1201     GTGAACTCTG TGATTGAGAA GATGAACACC CAGTTCACAG CAGTGGGCAA GGAGTTCAAC
1261     CAACTTGAGA GGAGGATTGA GAACCTGAAC AAGAAGGTGG ATGATGGCTT CCTGGACATC
1321     TGGACCTACA ATGCTGAACT GCTGGTGCTG TTGGAGAATG AGAGGACCCT GGACTACCAT
1381     GACAGCAATG TGAAGAACCT CTATGAGAAG GTGAGGAGCC AACTTAAAAA CAATGCCAAG
1441     GAGATTGGCA ATGGCTGTTT TGAGTTCTAC CACAAGTGTG ATGACACTTG TATGGAGTCT
1501     GTGAAGAATG GCACCTATGA CTACCCAAAA TACTCTGAGG AGGCTAAACT GCACAGGGAG
1561     GAGATTGATG GAGTGAAATT GGAGAGCACC AGGATTTACC AGATTCTGGC TATCTACAGC
1621     ACAGTGGCAT CCTCCCTGGT GCTGGTGGTG TCCCTGGGAG CCATCTCCTT CTGGATGTGT
1681     AGCAATGGCT CCCTCCAATG TAAGATTTGT ATTTAAATCT AGA

 

Quality Control:cDNA sequencing results is available upon request.