Influenza A H1N2 HA / Hemagglutinin Antibody (Antigen Affinity Purified)

H1N2 HA / Hemagglutinin Antibody ( Antigen Affinity Purified ) Datasheet

H1N2 HA / Hemagglutinin Antibody Product Information

• Immunogen: Recombinant H1N2 (A/swine/Guangxi/13/2006) HA / Hemagglutinin protein (Catalog#11703-V08H)
• Antibody Type: Rabbit Polyclonal Antibody ( Antibody Purification Platform )
• Ig Type: Rabbit IgG
• Formulation: 0.2 μm filtered solution in PBS with 5% trehalose
• Preparation: Produced in rabbits immunized with purified, recombinant H1N2 (A/swine/Guangxi/13/2006) HA / Hemagglutinin (Catalog#11703-V08H; ABQ42444.1; Met 1-Gln 529). H1N2 (A/swine/Guangxi/13/2006) HA / Hemagglutinin specific IgG was purified by H1N2 (A/swine/Guangxi/13/2006) HA / Hemagglutinin affinity chromatography.

H1N2 HA / Hemagglutinin Antibody Usage Guide

• Specificity: H1N2 (A/swine/Guangxi/13/2006) HA / Hemagglutinin
• Western blot: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect H1N2 (A/swine/Guangxi/13/2006) HA in WB. Using a DAB detection system, the detection limit for H1N2 (A/swine/Guangxi/13/2006) HA is approximately 0.5 ng/lane under non-reducing conditions and 1 ng/lane under reducing conditions.
• Direct ELISA: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect H1N2 (A/swine/Guangxi/13/2006) HA. The detection limit for H1N2 (A/swine/Guangxi/13/2006) HA is approximately 0.00245 ng/well.
• Storage: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
  Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

H1N2 HA / Hemagglutinin Antibody Background

Influenza (flu) is a respiratory infection in mammals and birds. This virus is divided into three main types (A, B and C). Influenza A is found in a wide variety of bird and mammal species and is further divided into subtypes based on differences in the membrane proteins hemagglutinin (HA) and neuraminidase (NA). H1N1, H1N2, and H3N2 are the only known Influenza A virus subtypes currently circulating among humans. H1N2 is currently pandemic in both human and pig populations. H1N2 does not cause more severe illness than other influenza viruses, and no unusual increases in influenza activity have been associated with it. Between December 1988 and March 1989, 19 influenza H1N2 virus isolates were identified in 6 cities in China, but the virus did not spread further. H1N2 was identified during the 2001-2002 flu season in Canada, the USA., Ireland, Latvia, France, Romania, Oman, India, Malaysia, and Singapore with earliest documented outbreak of the virus occurring in India on May 31, 2001. On February 6, 2002, the World Health Organization (WHO) in Geneva and the Public Health Laboratory Service in the United Kingdom reported the identification influenza A H1N2 virus from humans in the UK, Israel, and Egypt. The 2001-2002 Influenza A H1N2 Wisconsin strain appears to have resulted from the reassortment of the genes of the currently circulating influenza A H1N1 and influenza A H3N2 subtypes. Hemagglutinin (HA) is a single-pass type I integral membrane glycoprotein from the influenza virus, and comprises over 80% of the envelope proteins present in the virus particle. The HA is a trimer with a receptor binding pocket on the globular head of each monomer. In natural infection, inactive HA is matured into HA1 and HA2 outside the cell by one or more trypsin-like, arginine-specific endoprotease secreted by the bronchial epithelial cells. Binding of HA to sialic acid-containing receptors on the surface of its target cell brings about the attachment of the virus particle to the cell and forms a endosome. Low pH in endosomes induce an irreversible conformational change in HA2, releasing the hydrophobic portion "fusion peptide". After which, virus penetrates the cell and pours its contents including the RNA genome into the cytoplasm mediated by fusion of the endocytosed virus particle's own membrane and the endosomal membrane. Hemagglutinin plays a major role in the determination of host range restriction and virulence.

References

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