Influenza A H1N1 (A/Puerto Rico/8/34/Mount Sinai) Nucleoprotein Gene cDNA Clone / ORF Clone (codon optimized)

Influenza A H1N1 (A/Puerto Rico/8/34/Mount Sinai) Nucleoprotein cDNA Clone Datasheet

Influenza A H1N1 (A/Puerto Rico/8/34/Mount Sinai) Nucleoprotein cDNA Clone Product Information

• cDNA Size: 1470
• Gene Synonym: NP，Nucleoprotein
• Source: Influenza Virus

Influenza A H1N1 (A/Puerto Rico/8/34/Mount Sinai) Nucleoprotein cDNA Clone Description

• cDNA Description: ORF Clone of Influenza A H1N1 (A/Puerto Rico/8/34/Mount Sinai) Nucleoprotein DNA.

  This cDNA clone has gone through customized codon optimization in order to obtain high level of protein expression in particular cell lines. Therefore, although the translated amino acid sequence is identical to the amino sequence on Gene Bank, the DNA sequence is different from that on Gene Bank.

• Vector: pGEM-T Vector

Influenza A H1N1 (A/Puerto Rico/8/34/Mount Sinai) Nucleoprotein cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

Influenza A H1N1 (A/Puerto Rico/8/34/Mount Sinai) Nucleoprotein cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O . Ensure the filter paper is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95℃ for 15-30 min.
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the scattered filter paper from the elute. The elute now contains the purified DNA.

Influenza A H1N1 (A/Puerto Rico/8/34/Mount Sinai) Nucleoprotein cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGCGTCTC AAGGCACCAA ACGATCTTAC GAACAGATGG AGACTGATGG AGAACGCCAG
61       AATGCCACTG AAATCAGAGC ATCCGTCGGA AAAATGATTG GTGGAATTGG ACGATTCTAC
121      ATCCAAATGT GCACCGAACT CAAACTCAGT GATTATGAGG GACGGTTGAT CCAAAACAGC
181      TTAACAATAG AGAGAATGGT GCTCTCTGCT TTTGACGAAA GGAGAAATAA ATACCTTGAA
241      GAACATCCCA GTGCGGGGAA AGATCCTAAG AAAACTGGAG GACCTATATA CAGGAGAGTA
301      AACGGAAAGT GGATGAGAGA ACTCATCCTT TATGACAAAG AAGAAATGAG GCGAATCTGG
361      CGCCAAGCTA ATAATGGTGA CGATGCAACG GCTGGTCTGA CTCACATGAT GATCTGGCAT
421      TCCAATTTGA ATGATGCAAC TTATCAGAGG ACAAGAGCTC TTGTTCGCAC CGGAATGGAT
481      CCCAGGATGT GCTCTCTGAT GCAAGGTTCA ACTCTCCCTA GGAGGTCTGG AGCCGCAGGT
541      GCTGCAGTCA AAGGAGTTGG AACAATGGTG ATGGAATTGG TCAGAATGAT CAAACGTGGG
601      ATCAATGATC GGAACTTCTG GAGGGGTGAG AATGGACGAA AAACAAGAAT TGCTTATGAA
661      AGAATGTGCA ACATTCTCAA AGGGAAATTT CAAACTGCTG CACAAAAAGC AATGATGGAT
721      CAAGTGAGAG AGAGCCGGAA CCCAGGGAAT GCTGAGTTCG AAGATCTCAC TTTTCTAGCA
781      CGGTCTGCAC TCATATTGAG AGGGTCGGTT GCTCACAAGT CCTGCCTGCC TGCCTGTGTG
841      TATGGACCTG CCGTAGCCAG TGGGTACGAC TTTGAAAGGG AGGGATACTC TCTAGTCGGA
901      ATAGACCCTT TCAGACTGCT TCAAAACAGC CAAGTGTACA GCCTAATCAG ACCAAATGAG
961      AATCCAGCAC ACAAGAGTCA ACTGGTGTGG ATGGCATGCC ATTCTGCCGC ATTTGAAGAT
1021     CTAAGAGTAT TAAGCTTCAT CAAAGGGACG AAGGTGCTCC CAAGAGGGAA GCTTTCCACT
1081     AGAGGAGTTC AAATTGCTTC CAATGAAAAT ATGGAGACTA TGGAATCAAG TACACTTGAA
1141     CTGAGAAGCA GGTACTGGGC CATAAGGACC AGAAGTGGAG GAAACACCAA TCAACAGAGG
1201     GCATCTGCGG GCCAAATCAG CATACAACCT ACGTTCTCAG TACAGAGAAA TCTCCCTTTT
1261     GACAGAACAA CCATTATGGC AGCATTCAAT GGGAATACAG AGGGGAGAAC ATCTGACATG
1321     AGGACCGAAA TCATAAGGAT GATGGAAAGT GCAAGACCAG AAGATGTGTC TTTCCAGGGG
1381     CGGGGAGTCT TCGAGCTCTC GGACGAAAAG GCAGCGAGCC CGATCGTGCC TTCCTTTGAC
1441     ATGAGTAATG AAGGATCTTA TTTCTTCGGA

 

Quality Control:cDNA sequencing results is available upon request.