Influenza A Nucleoprotein / NP Antibody

H1N1 Nucleoprotein / NP Antibody Datasheet

H1N1 Nucleoprotein / NP Antibody Product Information

• Immunogen: Recombinant H1N1 NP protein (Catalog#11675-V08B)
• Antibody Type: Rabbit Polyclonal Antibody ( Antibody Purification Platform )
• Ig Type: Rabbit IgG
• Formulation: 0.2 μm filtered solution in PBS with 5% trehalose
• Preparation: Produced in rabbits immunized with purified, human cell-derived, recombinant Influenza A virus H1N1 Nucleoprotein / NP. Total IgG was purified by protein A affinity chromatography.

H1N1 Nucleoprotein / NP Antibody Usage Guide

• Specificity: Influenza A H1N1 Nucleoprotein
• Western blot: This antibody can be used at 1-2 μg/mL with the appropriate secondary reagents to detect H1N1 NP in WB.
• Direct ELISA: This antibody can be used at 0.5-1 μg/mL with the appropriate secondary reagents to detect H1N1 NP. The detection limit for H1N1 NP is approximately 0.00975 ng/well.
• Storage: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
  Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

H1N1 Nucleoprotein / NP Antibody Background

Influenza (flu) is a respiratory infection in mammals and birds. This virus is divided into three main types (A, B and C). Influenza A is found in a wide variety of bird and mammal species. Influenza B is largely confined to humans and is an important cause of morbidity. Influenza C infects humans, dogs and pigs, sometimes causing both severe illness and local epidemics. Influenza A is further divided into subtypes based on differences in the membrane proteins hemagglutinin (HA) and neuraminidase (NA). The notation HhNn is used to refer to the subtype comprising the hth discovered HA protein and the nth discovered NA protein. Influenza viruses A, B and C are very similar in overall structure. The viral particles of all influenza viruses are similar in composition. These are made of a viral envelope containing two main types of glycoproteins, wrapped around a central core. The central core contains the viral RNA genome and other viral proteins that package and protect this RNA. The influenza A genome contains 11 genes on eight pieces of RNA, encoding for 11 proteins: Hemagglutinin (HA), Neuraminidase (NA), Nucleoprotein (NP), M1, M2, NS1, NS2(NEP), PA, PB1, PB1-F2 and PB2.
Influenza A virus nucleoprotein (NP) forms homo-oligomers and multiple copies of NP wrap around genomic RNA, along with a trimeric polymerase making up ribonucleoprotein (RNP) complex. NP is composed of a head and a body domain and a tail loop / linker region. The head domain is more conserved than the body domain. NP oligomerization is mediated by the insertion of the non-polymorphic and structurally conserved tail loop of one NP molecule to a groove of another NP. The different form of NP oligomers is due to the flexibility of the polymorphic linkers that join the tail loop to the rest of the protein. The RNA binding property of NP is known to involve the protruding element and the flexible basic loop between the head and body domains, both having high degree of primary sequence conservation.

References

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2. Epstein, S.L. et al., 2005, Vaccine. 23 (46-47): 5404-10.
3. Christophe F, et al., 2009, Science. 324: 1557-61.
4. Guan Y, et al., 2009, Revue scientifique et technique. 23(2): 453-65.
5. Ng, A.K. et al., 2009, Sci China C Life Sci. 52 (5):439-49.