Influenza A H1N1 ( A/California/04/2009 ) NA / Neuraminidase Protein (Active)

H1N1 ( A/California/04 /2009 ) NA / Neuraminidase Protein

H1N1 NA / Neuraminidase Protein Product Information

• Synonym: NA
• Protein Construction: A DNA sequence encoding the mature form of influenza A virus ( A/California/04/2009 ( H1N1 ) ) neuraminidase ( ACP41107.1 ) ( His 36 -Lys 469 ) was fused with a polyhistidine tag at the N-terminus
• Source: Influenza A Virus H1N1
• Expression Host: Baculovirus-Insect cells

H1N1 NA / Neuraminidase Protein QC Testing

• Purity: > 88 % as determined by SDS-PAGE
• Bio-activity: Measured by its ability to cleave a fluorogenic substrate, 2’-(4-Methylumbelliferyl)-α-D-N-acetylneuraminic acid
  The specific activity is 800-1200 pmoles/min/µg
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: His
• Molecular Mass: The recombinant influenza H1N1 virus neuraminidase ( A/California/04/2009 ( H1N1 ) ) comprises 450 amino acids with the predicted molecular mass of 50 kDa. The apparent molecular mass of the recombinant protein is approximately 55 kDa in SDS-PAGE under reducing conditions
• Formulation: Lyophilized from sterile 20 mM Tris, 500 mM NaCl, pH 7.4 , 10% gly
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: H1N1 NA protein

H1N1 NA / Neuraminidase Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Related Influenza Virus Research Tools

• Influenza Virus Research Tools
• Influenza Neuraminidase & Antibody
• Influenza Neuraminidase (NA) Protein
• H1N1 Neuraminidase
• A/California/04/2009

H1N1 NA / Neuraminidase Protein Description

Neuraminidase ( NA ) and hemagglutinin ( HA ) are major membrane glycoproteins found on the surface of influenza virus. NA, also called sialidases, specifically catalyze the hydrolysis removal of terminal sialic acid residues from viral and cellular glycoconjugates. It is known that HA binds to the sialic acid-containing receptors on the surface of host cells during initial infection, and at the end of an infectious cycle, NA cleaves the HA-sialic acid bondage from the newly formed virions and the host cell receptors during budding. NA thus is described as a receptor-destroying enzyme which facilitates virus release and efficient spread of the progeny virus from cell to cell. NA is a single-pass type II membrane protein which exists as a homotetramer, and the transmembrane domain is involved in lipid raft association during intracellular transport. NA is suggested to play a role in the determination of host range restriction on replication and virulence. Nine subtypes of NA have been identified, and subtypes N1 and N2 have been positively linked to epidemics in man.

References

1. Barman, S. and Nayak, D.P. 2000, J. Virol. 74: 6538-6545
2. Colman, P.M. et al., 1983, Nature. 303: 41-44
3. Suzuki, T. et al., 2005, J. Virol. 79: 11705-11715
4. von, Itzstein, M. 2007, Nat. Rev. Drug. Discov. 6: 967-974