OSMR / IL-31RB Antibody (Cytokine)

OSMR / IL-31RB Antibody Datasheet

OSMR / IL-31RB Antibody Product Information

• Immunogen: Recombinant Human OSMR / IL-31RB Protein (Catalog#11226-H08H)
• Antibody Type: Rabbit Monoclonal Antibody ( Rabbit mAb Service Platform )
• Clone ID: 007
• Ig Type: Rabbit IgG
• Formulation: 0.2 μm filtered solution in PBS with 5% trehalose
• Preparation: This antibody was obtained from a rabbit immunized with purified, human cell-derived, recombinant Human OSMR / IL-31RB (rh OSMR / IL-31RB; Catalog#11226-H08H; NP_003990.1; Met 1 - Met 740)

OSMR / IL-31RB Antibody Usage Guide

• Specificity: Human OSMR / IL-31RB
• Western blot: This antibody can be used at 1-2 μg/mL with the appropriate secondary reagents to detect Human OSMR in WB.Using a DAB detection system, the detection limit for Human OSMR is approximately 1 ng/lane under non-reducing conditions and 20 ng/lane under reducing conditions.
• Direct ELISA: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human OSMR. The detection limit for Human OSMR is approximately 0.00245 ng/well.
• Storage: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
  Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

OSMR / IL-31RB Antibody Background

Oncostatin-M-specific receptor subunit beta, also known as interleukin-31 receptor subunit beta, and OSMR, is a single-pass type I  membrane protein which belongs to the type I  cytokine receptor family and Type 2 subfamily. OSMR contains four fibronectin type-III domains and is expressed at relatively high levels in all neural cells as well as fibroblast, epithelial and a variety of tumor cell lines. Four forms of OSMR have been identified: leukemia inhibitory factor receptor (LIFR), OSMR beta, short-form OSMR (OSMRs) and soluble OSMR (sOSMR). OSMR associates with IL31RA to form the IL31 receptor. It binds IL31 to activate STAT3 and possibly STAT1 and STAT5. OSMR is part of a heterodimeric receptor complex that mediates signal transduction of the pleiotropic cytokine OSM via a signaling pathway involving Janus kinases (Jaks) and transcription factors of the signal transducers and activators of transcription (STAT) family. Defects in OSMR are the cause of amyloidosis type 9 (AMYL9) which is a hereditary primary amyloidosis characterized by localized cutaneous amyloid deposition.

References

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5. Chen, D. et al., 2008, J Pathol. 215 (3): 290-9.