Influenza A H1N1 (Swine Flu 2009) NA / Neuraminidase Antibody

Influenza A H1N1 ( Swine Flu 2009 ) Neuraminidase Antibody Datasheet

Influenza A H1N1 ( Swine Flu 2009 ) Neuraminidase Antibody Product Information

• Immunogen: Recombinant H1N1 NA Protein
• Antibody Type: Rabbit Monoclonal Antibody ( Rabbit mAb Service Platform )
• Clone ID: 001
• Ig Type: Rabbit IgG
• Formulation: 0.2 μm filtered solution in PBS with 5% trehalose
• Preparation: This antibody was obtained from a rabbit immunized with purified, human cell-derived, recombinant Influenza A virus H1N1 Hemagglutinin

Influenza A H1N1 ( Swine Flu 2009 ) Neuraminidase Antibody Usage Guide

• Specificity: H1N1 (A/California/04/2009) NA
• Western blot: This antibody can be used at 1-2 μg/mL with the appropriate secondary reagents to detect H1N1-C-NA in WB. Using a DAB detection system, the detection limit for H1N1-C-NA is approximately 0.25 ng/lane under non-reducing conditions and 10 ng/lane under reducing conditions.
• Direct ELISA: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect H1N1-C-NA. The detection limit for H1N1-C-NA is approximately 0.00245 ng/well.
• Storage: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
  Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

Influenza A H1N1 ( Swine Flu 2009 ) Neuraminidase Antibody Background

Neuraminidase ( NA ) and hemagglutinin ( HA ) are major membrane glycoproteins found on the surface of influenza virus. NA, also called sialidases, specifically catalyze the hydrolysis removal of terminal sialic acid residues from viral and cellular glycoconjugates. It is known that HA binds to the sialic acid-containing receptors on the surface of host cells during initial infection, and at the end of an infectious cycle, NA cleaves the HA-sialic acid bondage from the newly formed virions and the host cell receptors during budding. NA thus is described as a receptor-destroying enzyme which facilitates virus release and efficient spread of the progeny virus from cell to cell. NA is a single-pass type II membrane protein which exists as a homotetramer, and the transmembrane domain is involved in lipid raft association during intracellular transport. NA is suggested to play a role in the determination of host range restriction on replication and virulence. Nine subtypes of NA have been identified, and subtypes N1 and N2 have been positively linked to epidemics in man.

References

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2. Colman, P.M. et al., 1983, Nature. 303: 41-44
3. Suzuki, T. et al., 2005, J. Virol. 79: 11705-11715
4. von, Itzstein, M. 2007, Nat. Rev. Drug. Discov. 6: 967-974