Influenza A H1N1 (A/California/04/2009) Hemagglutinin Gene cDNA Clone / ORF Clone (Codon Optimized)

Influenza A H1N1 (A/California/04/2009) Hemagglutinin cDNA Clone Datasheet

Influenza A H1N1 (A/California/04/2009) Hemagglutinin cDNA Clone Product Information

• cDNA Size: 1722
• Gene Synonym: HA1, Hemagglutinin
• Source: Influenza Virus

Influenza A H1N1 (A/California/04/2009) Hemagglutinin cDNA Clone Description

• cDNA Description: ORF Clone of Influenza A H1N1 (A/California/04/2009) HA DNA.

  This cDNA clone has gone through customized codon optimization in order to obtain high level of protein expression in particular cell lines. Therefore, although the translated amino acid sequence is identical to the amino sequence on Gene Bank, the DNA sequence is different from that on Gene Bank.

• Vector: Cloning Vector: pGEM-T Vector. Restriction site: HidIII + XbaI

  Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG

Influenza A H1N1 (A/California/04/2009) Hemagglutinin cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

Influenza A H1N1 (A/California/04/2009) Hemagglutinin cDNA Clone Usage Protocol

1. Cut along the circle edge of the spot (arrow points to) to get the filter paper.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O . Ensure the filter paper is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to incubation at 95℃ for 15-30 min.
4. At the end of the incubation pulse vortex, or gently tap the tube.
5. Briefly centrifuge for 30 seconds, to separate the scattered filter paper from the elute. The elute now contains the purified DNA.

Influenza A H1N1 (A/California/04/2009) Hemagglutinin cDNA Clone Sequence and Quality Control

Sequence:

1        AAGCTTGCCG CCACCATGAA GGCTATCCTG GTGGTGCTGC TCTACACCTT TGCCACAGCC
61       AATGCTGACA CCCTGTGTAT TGGCTACCAT GCCAACAACA GCACAGACAC AGTGGACACA
121      GTGTTGGAGA AGAATGTGAC AGTGACCCAC TCTGTGAACC TGTTGGAGGA CAAACACAAT
181      GGCAAACTGT GTAAACTGAG GGGAGTGGCT CCACTGCACC TGGGCAAGTG TAACATTGCT
241      GGCTGGATTC TGGGCAACCC TGAGTGTGAG TCCCTGAGCA CAGCCTCCTC CTGGTCCTAC
301      ATTGTGGAGA CACCATCCTC TGACAATGGC ACTTGTTACC CTGGAGACTT CATTGACTAT
361      GAGGAACTGA GGGAACAACT TTCCTCTGTG TCCTCCTTTG AGAGGTTTGA GATTTTTCCA
421      AAGACCTCCT CCTGGCCAAA CCATGACAGC AACAAGGGAG TGACAGCAGC CTGTCCACAT
481      GCTGGAGCCA AGTCCTTCTA CAAGAACCTG ATTTGGCTGG TGAAGAAGGG CAACTCCTAC
541      CCAAAACTGA GCAAGTCCTA CATCAATGAC AAGGGCAAGG AGGTGCTGGT GCTGTGGGGC
601      ATCCACCACC CAAGCACCTC TGCTGACCAA CAGTCCCTCT ACCAGAATGC TGACACCTAT
661      GTGTTTGTGG GCTCCAGCAG ATACAGCAAG AAGTTCAAGC CTGAGATTGC CATCAGACCA
721      AAGGTGAGGG ACCAGGAGGG CAGGATGAAC TACTACTGGA CCCTGGTGGA ACCTGGAGAC
781      AAGATTACCT TTGAGGCTAC AGGCAACCTG GTGGTGCCAA GATATGCCTT TGCTATGGAG
841      AGGAATGCTG GCTCTGGCAT CATCATCTCT GACACACCTG TCCATGACTG TAACACCACT
901      TGTCAGACAC CAAAGGGAGC CATCAACACC TCCCTGCCAT TCCAGAACAT CCACCCAATC
961      ACCATTGGCA AGTGTCCAAA ATATGTGAAG AGCACCAAAC TGAGACTGGC TACAGGACTG
1021     AGGAACATCC CAAGCATCCA GAGCAGGGGA CTGTTTGGAG CCATTGCTGG CTTCATTGAG
1081     GGAGGCTGGA CAGGGATGGT GGATGGCTGG TATGGCTACC ACCACCAGAA TGAACAGGGC
1141     TCTGGCTATG CTGCTGACCT GAAAAGCACC CAGAATGCCA TTGATGAGAT TACCAACAAG
1201     GTGAACTCTG TGATTGAGAA GATGAACACC CAGTTCACAG CAGTGGGCAA GGAGTTCAAC
1261     CACTTGGAGA AGAGGATTGA GAACCTGAAC AAGAAGGTGG ATGATGGCTT CCTGGACATC
1321     TGGACCTACA ATGCTGAACT GCTGGTGCTG TTGGAGAATG AGAGGACCCT GGACTACCAT
1381     GACAGCAATG TGAAGAACCT CTATGAGAAG GTGAGGAGCC AACTTAAAAA CAATGCCAAG
1441     GAGATTGGCA ATGGCTGTTT TGAGTTCTAC CACAAGTGTG ACAACACTTG TATGGAGTCT
1501     GTGAAGAATG GCACCTATGA CTACCCAAAA TACTCTGAGG AGGCTAAACT GAACAGGGAG
1561     GAGATTGATG GAGTGAAATT GGAGAGCACC AGGATTTACC AGATTTTGGC GATCTATTCA
1621     ACTGTCGCCA GTTCATTGGT ACTGGTAGTC TCCCTGGGGG CAATCAGTTT CTGGATGTGC
1681     TCTAATGGGT CTCTACAGTG TAGAATATGT ATTTAATCTA GA

 

Quality Control:cDNA sequencing results is available upon request.