Influenza A H1N1 (A/Beijing/22808/2009)HA Protein (His Tag)

Hemagglutinin (Influenza A virus (A/Beijing/22808/2009 (H1N1)) Protein Datasheet
 

H1N1(A/Beijing/22808/2009)HA Protein Product Information

• Synonym: HA
• Protein Construction: A DNA sequence encoding the extracellular domain of influenza A virus hemagglutinin (A/Beijing/22808/2009 (H1N1))（ADD64203.1）(Met 1-Gln 529) was fused with a polyhistidine tag at the C-terminus.
• Source: Influenza A Virus
• Expression Host: Human Cells

H1N1(A/Beijing/22808/2009)HA Protein QC Testing

• Purity: > 95 % as determined by SDS-PAGE.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Asp 18
• Molecular Mass: The secreted recombinant hemagglutinin of H1N1(A/Beijing/22808/2009)HA (A/Beijing/22808/2009 (H1N1)) comprises 523 amino acids and has a predicted molecular mass of 59 kDa. As a result of glycosylation, it migrates as an approximately 72 kDa band in SDS-PAGE under reducing conditions.
• Formulation: Lyophilized from sterile PBS , pH 7.4
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: H1N1(A/Beijing/22808/2009)HA protein

H1N1(A/Beijing/22808/2009)HA Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

H1N1(A/Beijing/22808/2009)HA Protein Description

Hemagglutinin (HA) is a single-pass type I integral membrane glycoprotein from the influenza virus, and comprises over 80% of the envelope proteins present in the virus particle. The HA is a trimer with a receptor binding pocket on the globular head of each monomer. In natural infection, inactive HA is matured into HA1 and HA2 outside the cell by one or more trypsin-like, arginine-specific endoprotease secreted by the bronchial epithelial cells. Binding of HA to sialic acid-containing receptors on the surface of its target cell brings about the attachment of the virus particle to the cell and forms a endosome. Low pH in endosomes induce an irreversible conformational change in HA2, releasing the hydrophobic portion "fusion peptide". After which, virus penetrates the cell and pours its contents including the RNA genome into the cytoplasm mediated by fusion of the endocytosed virus particle's own membrane and the endosomal membrane. Hemagglutinin plays a major role in the determination of host range restriction and virulence.

References

1. Barman S. et al., 2000, J Virol. 74: 6538-45.
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3. Shinya K. et al., 2006, Nature. 440 (7083): 435-6.
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5. Christophe F. et al., 2009, Science. 324: 1557-61.
6. Von Itzstein M. 2007, Nat Rev Drug Discov. 6: 967-74.