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Influenza A H1N1 (Swine Flu 2009) Hemagglutinin Antibody
|Order or Inquire for H1N1 HA Antibody product||Quality antibodies||Antibody production services|
|Detection limit is 10 ng/well in ELISA|
Influenza A H1N1 HA Antibody Product Information
Recombinant H1N1 HA protein
|Antibody Type :||Mouse Monoclonal Antibody ( Mouse mAb Service Platform )|
|Ig Type:||Mouse IgG2b|
|Formulation:||0.2 μm filtered solution in PBS with 5% trehalose|
|Preparation:||This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, human cell-derived, recombinant Influenza A virus H1N1 hemagglutinin (HA) extracellular domain. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.|
Influenza A H1N1 HA Antibody Usage Guide
H1N1 ( A/California/04/2009 ) HA
H1N1 ( A/California/07/2009 ) HA
Has cross-reactivity in ELISA with
H1N2 (A/swine/Guangxi/13/2006) HA
H1N3 (A/duck/NZL/160/1976) HA
H5N1 (A/Anhui/1/2005) HA
H5N1 (A/Viet nam/1194/2004) HA
H5N1 (A/Indonesia/5/2005) HA
H5N1 (A/turkey/Turkey/1/2005) HA
H5N1 (A/bar-headed goose/Qinghai/14/2008) HA
No cross-reactivity in WB and ELISA with
H1N1 (A/Brisbane/59/2007) HA
H1N1 (A/BrevigMission/1/1918) HA
H1N1 (A/Solomon Islands/3/2006) HA
H1N1 (A/Ohio/UR06-0091/2007) HA
H1N1 (A/New Caledonia/20/1999) HA
H1N1 (A/Puerto Rico/8/1934) HA
H1N1 (A/WSN/1933) HA
H3N2 (A/Brisbane/10/2007) HA
Influenza B (B/Florida/4/2006) HA
Human cell lysate ( 293 cell line )
|Flow Cytometry :||
High Five cells were infected by Bac-HA, cells were collected at 48 hours postinfection, and the cell surface expression of HA were measured by flow cytometry. 106 Cells were stained with 1 μg Purified Mouse Anti-H1N1-HA (11055-MM08) antibody for 20 min on ice. Cells were washed twice and incubated with 1 μg of a FITC Goat Anti-Mouse Ig secondary antibody for 20 min on ice. Cells were washed twice and analyzed by flow cytometry.
Flow cytometry was performed on a BD FACSCalibur flow cytometry system
Please refer to www.sinobiological.com/Flow-Cytometry-FACS-Protocols-a-750.html for technical protocols.
|Western blot:||This antibody can be used at 1-2 μg/mL with the appropriate secondary reagents to detect H1N1 HA in WB|
|Direct ELISA:||This antibody can be used at 0.5 - 1 μg/mL with the appropriate secondary reagents to detect H1N1 HA. The detection limit for H1N1 HA is approximately 10ng/well|
|Storage:||This antibody can be stored at 2℃-8℃ for 1 month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Influenza A H1N1 HA Antibody Background
The H1N1 viral strain ( A/California/04/2009 ) implicated in the 2009 flu pandemic among humans is often called "swine flu" because initial testing showed many of the genes in the virus were similar to influenza viruses normally occurring in North American swine. Two children in southern California were infected with H1N1 swine flu. The isolates are similar and have an unusual constellation of genes. The lack of contact between the two children, as well as a lack of contact with swine, suggests the virus is spreading human to human. The presence of swine H1N1 in humans raises concerns of recombination with H1N1 seasonal flu. Moreover, the 1918 pandemic strain was a recombinant between human H1N1 and swine H1N1. The likely ability of this swine H1N1 to transmit efficiently in humans is cause for concern. The HA, NA, and MP sequences of A/California/04/2009 have been placed on deposit at GISAID.
Related Influenza Virus Research Tools
- Li KS, et al., 2004, Nature. 430: 209-13.
- The Writing Committee of the WHO Consultation on Human Influenza A/H5, 2005, N Engl J Med 353 (13): 1374-85.
- Taubenberger JK, et al., 2008. Annu Rev Pathol. 3: 499-522.
- Christophe F, et al., 2009, Science. 324:1557-1561.
- Guan Y, et al., 2009, Revue scientifique et technique. 23(2): 453-65.
- Morbidity and Mortality Weekly Report. 2009-04-24. p. 400-402.
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|Protein :||hemagglutinin (HA), neuraminidase (NA), nucleoprotein (NP), matrix protein 1 (M1), nonstructural protein 1 (NS1), nonstructural protein 2 (NS2), influenza antigen (influenza protein)|
|ELISA Kit :||Influenza ELISA Kit|
|Gene :||Influenza Gene / Influenza cDNA Clone|
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