Influenza A H1N1 (Swine Flu 2009) HA Antibody

Influenza A H1N1 (Swine Flu 2009) Hemagglutinin Antibody

Influenza A H1N1 HA Antibody Product Information

• Immunogen: Recombinant H1N1 HA protein
• Antibody Type: Mouse Monoclonal Antibody ( Mouse mAb Service Platform )
• Clone ID: 9G1G8
• Ig Type: Mouse IgG2b
• Formulation: 0.2 μm filtered solution in PBS with 5% trehalose
• Preparation: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, human cell-derived, recombinant Influenza A virus H1N1 hemagglutinin (HA) extracellular domain. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.

Influenza A H1N1 HA Antibody Usage Guide

• Specificity: H1N1 ( A/California/04/2009 ) HA

  H1N1 ( A/California/07/2009 ) HA

• Has cross-reactivity in ELISA with

  H1N2 (A/swine/Guangxi/13/2006) HA

  H1N3 (A/duck/NZL/160/1976) HA

  H5N1 (A/Anhui/1/2005) HA

  H5N1 (A/Viet nam/1194/2004) HA

  H5N1 (A/Indonesia/5/2005) HA

  H5N1 (A/turkey/Turkey/1/2005) HA

  H5N1 (A/bar-headed goose/Qinghai/14/2008) HA

  No cross-reactivity in WB and ELISA with

  H1N1 (A/Brisbane/59/2007) HA

  H1N1 (A/BrevigMission/1/1918) HA

  H1N1 (A/Solomon Islands/3/2006) HA

  H1N1 (A/Ohio/UR06-0091/2007) HA

  H1N1 (A/New Caledonia/20/1999) HA

  H1N1 (A/Puerto Rico/8/1934) HA

  H1N1 (A/WSN/1933) HA

  H3N2 (A/Brisbane/10/2007) HA

  Influenza B (B/Florida/4/2006) HA

  Human cell lysate ( 293 cell line )

• Flow Cytometry:
  

  High Five cells were infected by Bac-HA, cells were collected at 48 hours postinfection, and the cell surface expression of HA were measured by flow cytometry. 10⁶ Cells were stained with 1 μg Purified Mouse Anti-H1N1-HA (11055-MM08) antibody for 20 min on ice. Cells were washed twice and incubated with 1 μg of a FITC Goat Anti-Mouse Ig secondary antibody for 20 min on ice. Cells were washed twice and analyzed by flow cytometry.

  Flow cytometry was performed on a BD FACSCalibur flow cytometry system

  Please refer to www.sinobiological.com/Flow-Cytometry-FACS-Protocols-a-750.html for technical protocols.

• Western blot: This antibody can be used at 1-2 μg/mL with the appropriate secondary reagents to detect H1N1 HA in WB
• Direct ELISA: This antibody can be used at 0.5 - 1 μg/mL with the appropriate secondary reagents to detect H1N1 HA. The detection limit for H1N1 HA is approximately 10ng/well
• Storage: This antibody can be stored at 2℃-8℃ for 1 month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
  Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

• Cross-reactivity of H1N1 (Swine Flu 2009) mAbs
  	Mouse		Rabbit		Mouse
  Catalog Number	11055-MM01	11055-MM04	11055-RM05	11055-RM06	11055-MM08	11055-MM09
  H1N1,A/California/07/2009	+	+	+	+	+	+
  H1N1,A/California/04/2009	+	+	+	+	+	+
  H1N1,A/California/04/2009, HA1 Sub-unit			+	－
  H1N1,A/Brisbane/59/2007	－	－	－	－	－	+
  H1N1,A/BrevigMission/1/1918			－	－
  H5N1,A/Anhui/1/2005	－	－	－	－	+	－
  H5N1,A/Anhui/1/2005, HA2 Sub-unit			－	+
  H5N1,A/Turkey/1/2005	－	－	－	+	+	－
  H5N1,A/bar-headed goose/ Qinghai/14/2008	－	－	－	－	+	－
  H5N1,A/Indonesia/5/2005	－	－	－	－	+	－
  H5N1,A/VietNam/1194/2004	－	－			+	－
  H5N1,A/VietNam/1203/2004			－	+
  H3N2,A/Brisbane/10/2007	－	－	－	－	－	－
  Influenza B, B/Florida/4/2006	－	－	－	－	－	－

Influenza A H1N1 HA Antibody Background

Influenza (flu) is a respiratory infection in mammals and birds. This virus is divided into three main types (A, B and C). Influenza A is found in a wide variety of bird and mammal species. Influenza B is largely confined to humans and is an important cause of morbidity. Influenza C infects humans, dogs and pigs, sometimes causing both severe illness and local epidemics. Influenza A is further divided into subtypes based on differences in the membrane proteins hemagglutinin (HA) and neuraminidase (NA). The notation HhNn is used to refer to the subtype comprising the hth discovered HA protein and the nth discovered NA protein. The HA is a trimer with a receptor binding pocket on the globular head of each monomer. Subtypes are further divided into strains. Each genetically distinct virus isolate is usually considered to be a separate strain.
The H1N1 viral strain ( A/California/04/2009 ) implicated in the 2009 flu pandemic among humans is often called "swine flu" because initial testing showed many of the genes in the virus were similar to influenza viruses normally occurring in North American swine. Two children in southern California were infected with H1N1 swine flu. The isolates are similar and have an unusual constellation of genes. The lack of contact between the two children, as well as a lack of contact with swine, suggests the virus is spreading human to human. The presence of swine H1N1 in humans raises concerns of recombination with H1N1 seasonal flu. Moreover, the 1918 pandemic strain was a recombinant between human H1N1 and swine H1N1. The likely ability of this swine H1N1 to transmit efficiently in humans is cause for concern. The HA, NA, and MP sequences of A/California/04/2009 have been placed on deposit at GISAID.

• Influenza Virus Research Tools
• Influenza Hemagglutinin & Antibody
• Influenza Antibodies
• H1N1 Hemagglutinin & Antibody
• H1N1 Hemagglutinin Antibodies
• A/California/04/2009

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