Influenza A H1N1 HA / Hemagglutinin Antibody

H1N1 HA Antibody Datasheet

H1N1 HA Antibody Product Information

• Immunogen: Recombinant H1N1 (A/Puerto Rico/8/1934) HA protein (Catalog#11684-V08H)
• Antibody Type: Mouse Monoclonal Antibody ( Mouse mAb Service Platform )
• Clone ID: 2F1A7
• Ig Type: Mouse IgG1
• Formulation: 0.2 μm filtered solution in PBS with 5% trehalose
• Preparation: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant H1N1 (A/Puerto Rico/8/1934) HA (Catalog#11684-V08H; ABD77675.1; Met 1-Gln 528). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.

H1N1 HA Antibody Usage Guide

• Specificity: H1N1 (A/Puerto Rico/8/1934) HA
• No cross-reactivity in ELISA with

  H1N1 (A/California/07/2009) HA
  H1N1 (A/California/04/2009) HA
  H1N1 (A/California/04/2009) HA1
  H1N1 (A/Brisbane/59/2007) HA
  H1N1 (A/Brisbane/59/2007) HA1
  H1N1 (A/BrevigMission/1/1918) HA
  H1N1 (A/Solomon Islands/3/2006)
  H1N1 (A/Ohio/UR06-0091/2007) HA
  H1N1 (A/New Caledonia/20/1999) HA
  H1N1 (A/WSN/1933) HA
  H1N2 (A/swine/Guangxi/13/2006) HA
  H1N3 (A/duck/NZL/160/1976)
  H5N1 (A/Anhui/1/2005) HA
  H5N1 (A/Anhui/1/2005) HA2

• Western blot: This antibody can be used at 1-2 μg/mL with the appropriate secondary reagents to detect H1N1 (A/Puerto Rico/8/1934) HA in WB. Using a DAB detection system, the detection limit for H1N1 (A/Puerto Rico/8/1934) HA is approximately 8 ng/lane under non-reducing conditions.Use of this antibody under reducing conditions is not recommended.
• Direct ELISA: This antibody can be used at 0.5-1 μg/mL with the appropriate secondary reagents to detect H1N1 (A/Puerto Rico/8/1934) HA. The detection limit for H1N1 (A/Puerto Rico/8/1934) HA is approximately 2.5 ng/well.
• Storage: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
  Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

H1N1 HA Antibody Background

Influenza (flu) is a respiratory infection in mammals and birds. This virus is divided into three main types (A, B and C). Influenza A is found in a wide variety of bird and mammal species and is further divided into subtypes based on differences in the membrane proteins hemagglutinin (HA) and neuraminidase (NA). Influenza A H1N1 virus is a subtype of influenza A virus. Some strains of H1N1 are endemic in humans and cause a small fraction of all influenza-like illness and a small fraction of all seasonal influenza. H1N1 strains caused a few percent of all human flu infections in 2004-2005. Other strains of H1N1 are endemic in pigs (swine influenza) and in birds (avian influenza). H1N1 was the most common cause of human influenza (flu) in 2009. In June 2009, the World Health Organization declared the new strain of swine-origin H1N1 as a pandemic. This strain is often called swine flu by the public media. This novel virus spread worldwide and had caused about 17,000 deaths by the start of 2010. Hemagglutinin (HA) is a single-pass type I integral membrane glycoprotein from the influenza virus, and comprises over 80% of the envelope proteins present in the virus particle. In natural infection, inactive HA is matured into HA1 and HA2 outside the cell by one or more trypsin-like, arginine-specific endoprotease secreted by the bronchial epithelial cells. Binding of HA to sialic acid-containing receptors on the surface of its target cell brings about the attachment of the virus particle to the cell and forms a endosome. Low pH in endosomes induce an irreversible conformational change in HA2, releasing the hydrophobic portion "fusion peptide". After which, virus penetrates the cell and pours its contents including the RNA genome into the cytoplasm mediated by fusion of the endocytosed virus particle's own membrane and the endosomal membrane. Hemagglutinin plays a major role in the determination of host range restriction and virulence.

References

1. Gunther-Ausborn, S. et al., 2000, J. Virol. 74: 2714-2720.
2. Hoffman, LR. et al., 1997, J. Virol. 71: 8808-8820.
3. Shinya K, et al., 2006, Nature.440 (7083): 435-6.
4. Taubenberger JK, et al., 2008.Annu Rev Pathol. 3: 499-522.
5. Christophe F, et al., 2009, Science. 324:1557-1561.
6. Morbidity and Mortality Weekly Report. 2009-04-24. p. 400-402.