H1F0 / Histone H1 Protein (His Tag)

H1 histone family, member 0 Protein Datasheet

H1F0 / Histone H1 Protein Product Information

• Synonym: HSPD1，CPN60, GROEL, HLD4, HSP60, HSP65, HuCHA60, SPG13
• Protein Construction: A DNA sequence encoding the human H1F0 (P07305) (Met 1-Lys 194) was expressed, with a polyhistide tag at the N-terminus.
• Source: Human
• Expression Host: E.coli

H1F0 / Histone H1 Protein QC Testing

• Purity: > 92 % as determined by SDS-PAGE.
• Endotoxin: Please contact us for more information.
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Met
• Molecular Mass: The recombinant human H1F0 consisting of 205 amino acids and has a calculated molecular mass of 22.4KDa. It migrates as an approximately 27 kDa band in SDS-PAGE under reducing conditions.
• Formulation: Lyophilized from 0.2μm filtered solution of 50mM Tris, 600mM Nacl 1mM DTT pH8.5
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: H1F0 / Histone H1 protein

H1F0 / Histone H1 Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

H1F0 / Histone H1 Protein Description

H1 histone family, member 0 (H1F0) is a member of the H1 histone family of nuclear proteins which are a component of chromatin in eukaryotic cells. It's involved in maintaining the structure of chromatin by packing the "beads on a string" sub-structure into a high order structure. The lysine-rich H1 histone family in mammals includes eleven members. In higher eukaryotes all H1 variants have the same general structure, consisting of a central conserved globular domain and less conserved N-terminal and C-terminal tails. These tails are moderately conserved among species, but differ among variants, suggesting a specific function for each H1 variant. Studies on the role of particular subtypes at specific developmental stages in lower eukaryotes, but also in vertebrates suggest that specific subtypes of H1 participate in particular systems of gene regulation.

References

1. Ramakrishnan V. et al., 1993, Nature. 362 (6417): 219-23.
2. Happel N. et al., 2009, Gene. 431 (1-2): 1-12.
3. Izzo A. et al., 2008, Biol Chem. 389 (4): 333-43.