Influenza A H16N3 (A/black-headed gull/Sweden/5/99) Hemagglutinin Gene cDNA Clone / ORF Clone (Codon Optimized)

Influenza A H16N3 (A/black-headed gull/Sweden/5/99) cDNA Clone Datasheet

Influenza A H16N3 (A/black-headed gull/Sweden/5/99) cDNA Clone Product Information

• cDNA Size: 1701
• Gene Synonym: HA1, Hemagglutinin
• Source: Influenza Virus

Influenza A H16N3 (A/black-headed gull/Sweden/5/99) cDNA Clone Description

• cDNA Description: ORF Clone of Influenza A H16N3 (A/black-headed gull/Sweden/5/99) HA DNA.

  This cDNA clone has gone through customized codon optimization in order to obtain high level of protein expression in particular cell lines. Therefore, although the translated amino acid sequence is identical to the amino sequence on Gene Bank, the DNA sequence is different from that on Gene Bank.

• Vector: Vector 3. Restriction site: Hind III + XbaI

  Optional Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG

Influenza A H16N3 (A/black-headed gull/Sweden/5/99) cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

Influenza A H16N3 (A/black-headed gull/Sweden/5/99) cDNA Clone Usage Protocol

1. Cut along the circle edge of the spot (arrow points to) to get the filter paper.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O . Ensure the filter paper is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to incubation at 95℃ for 15-30 min.
4. At the end of the incubation pulse vortex, or gently tap the tube.
5. Briefly centrifuge for 30 seconds, to separate the scattered filter paper from the elute. The elute now contains the purified DNA.

Influenza A H16N3 (A/black-headed gull/Sweden/5/99) cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGTGATTA AGGTGCTCTA CTTCCTGATT GTGCTGCTGA GCAGATACAG CAAGGCTGAC
61       AAGATTTGTA TTGGCTACCT GAGCAACAAT GCCACAGACA CAGTGGACAC CCTGACAGAG
121      AATGGAGTGC CTGTGACCTC CTCTGTGGAC CTGGTGGAGA CCAACCACAC AGGCACCTAC
181      TGTTCCCTGA ATGGCATCAG CCCAATCCAC CTGGGAGACT GTTCCTTTGA GGGCTGGATT
241      GTGGGCAACC CATCCTGTGC CACCAACATC AACATCAGGG AGTGGTCCTA CCTGATTGAG
301      GACCCAAATG CCCCAAACAA ACTGTGTTTT CCTGGAGAAC TGGACAACAA TGGAGAACTG
361      AGACACCTGT TCTCTGGAGT GAACTCCTTC AGCAGGACAG AACTGATTAG CCCAAACAAG
421      TGGGGAGACA TCCTGGATGG AGTGACAGCC TCCTGTAGGG ACAATGGAGC CTCCTCCTTC
481      TACAGGAACC TGGTGTGGAT TGTGAAGAAC AAGAATGGCA AATACCCTGT GATTAAGGGA
541      GACTACAACA ACACCACAGG CAGGGATGTG CTGGTGCTGT GGGGCATCCA CCACCCTGAC
601      ACAGAGACCA CAGCCATCAA CCTCTATGCC AGCAAGAACC CATACACCCT GGTGAGCACC
661      AAGGAGTGGA GCAAGAGATA TGAATTGGAG ATTGGCACCA GGATTGGAGA TGGACAAAGG
721      TCCTGGATGA AACTCTACTG GCACCTGATG AGACCTGGAG AGAGGATAAT GTTTGAGAGC
781      AATGGAGGAC TGATTGCCCC AAGATATGGC TACATCATTG AGAAATATGG CACAGGCAGG
841      ATTTTCCAGT CTGGAGTGAG GATGGCTAAG TGTAACACCA AGTGTCAGAC CTCCCTGGGA
901      GGCATCAACA CCAACAAGAC CTTCCAGAAC ATTGAGAGGA ATGCCCTGGG AGACTGTCCA
961      AAATACATCA AGTCTGGACA ACTTAAACTG GCTACAGGAC TGAGGAATGT GCCATCTGTG
1021     GGAGAGAGGG GACTGTTTGG AGCCATTGCT GGCTTCATTG AGGGAGGCTG GCCTGGACTG
1081     ATAAATGGCT GGTATGGCTT CCAACACCAG AATGAACAGG GCACAGGCAT TGCTGCTGAC
1141     AAGGCAAGCA CCCAGAAGGC TATTGATGAG ATTACCACCA AGATAAACAA CATCATTGAG
1201     AAGATGAATG GCAACTATGA CAGCATCAGG GGAGAGTTCA ACCAGGTGGA GAAGAGGATA
1261     AATATGCTGG CTGACAGGGT GGATGATGCT GTGACAGACA TCTGGTCCTA CAATGCCAAA
1321     CTGCTGGTGC TGTTGGAGAA TGGCAGGACC CTGGACCTCC ATGATGCCAA TGTGAGGAAC
1381     CTCCATGACC AGGTGAAGAG GATTCTGAAA AGCAATGCCA TTGATGAGGG AGATGGCTGT
1441     TTCAACCTGC TGCACAAGTG TAATGACTCC TGTATGGAGA CCATCAGGAA TGGCACCTAC
1501     AACCATGAGG ACTACAGGGA GGAGAGCCAA CTTAAAAGAC AGGAGATTGA GGGCATCAAA
1561     CTGAAATCTG AGGACAATGT CTACAAGGTG CTGAGCATCT ACTCCTGTAT TGCCTCCAGC
1621     ATTGTGCTGG TGGGACTGAT TCTGGCTTTC ATAATGTGGG CTTGTAGCAA TGGCAACTGT
1681     AGGTTCAATG TGTGTATTTA A

 

Quality Control:cDNA sequencing results is available upon request.