|Recombinant H12N5 (A/green-winged teal/ALB/199/1991) HA / Hemagglutinin protein (Catalog#11718-V08H)|
|0.2 μm filtered solution in PBS|
|Produced in rabbits immunized with purified, recombinant H12N5 (A/green-winged teal/ALB/199/1991) HA / Hemagglutinin (Catalog#11718-V08H; ABB88110.1; Met 1-Lys 527). H12N5 (A/green-winged teal/ALB/199/1991) HA / Hemagglutinin specific IgG was purified by H12N5 (A/green-winged teal/ALB/199/1991) HA / Hemagglutinin affinity chromatography.|
WB: 0.1-0.2 μg/mL
ELISA: 0.1-0.2 μg/mL
This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect H12N5 (A/green-winged teal/ALB/199/1991) HA. The detection limit for H12N5 (A/green-winged teal/ALB/199/1991) HA is approximately 0.00245 ng/well.
The influenza viral Hemagglutinin (HA) protein is a homo trimer with a receptor binding pocket on the globular head of each monomer.HA has at least 18 different antigens. These subtypes are named H1 through H18.HA has two functions. Firstly, it allows the recognition of target vertebrate cells, accomplished through the binding to these cells' sialic acid-containing receptors. Secondly, once bound it facilitates the entry of the viral genome into the target cells by causing the fusion of host endosomal membrane with the viral membrane.The influenza virus Hemagglutinin (HA) protein is translated in cells as a single protein, HA0, or hemagglutinin precursor protein. For viral activation, hemagglutinin precursor protein (HA0) must be cleaved by a trypsin-like serine endoprotease at a specific site, normally coded for by a single basic amino acid (usually arginine) between the HA1 and HA2 domains of the protein. After cleavage, the two disulfide-bonded protein domains produce the mature form of the protein subunits as a prerequisite for the conformational change necessary for fusion and hence viral infectivity.