Influenza A H11N9 (A/mallard/Alberta/294/1977) Hemagglutinin Gene cDNA Clone / ORF Clone (codon optimized)

Influenza A H11N9 (A/mallard/Alberta/294/1977) cDNA Clone Datasheet

Influenza A H11N9 (A/mallard/Alberta/294/1977) cDNA Clone Product Information

• cDNA Size: 1720
• Gene Synonym: HA1, Hemagglutinin
• Source: Influenza Virus

Influenza A H11N9 (A/mallard/Alberta/294/1977) cDNA Clone Description

• cDNA Description: ORF Clone of Influenza A H11N9 (A/mallard/Alberta/294/1977) HA DNA.

  This cDNA clone has gone through customized codon optimization in order to obtain high level of protein expression in particular cell lines. Therefore, although the translated amino acid sequence is identical to the amino sequence on Gene Bank, the DNA sequence is different from that on Gene Bank.

• Vector: Vector 3. Restriction site: Hind III + XbaI

Influenza A H11N9 (A/mallard/Alberta/294/1977) cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

Influenza A H11N9 (A/mallard/Alberta/294/1977) cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O . Ensure the filter paper is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95℃ for 15-30 min.
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the scattered filter paper from the elute. The elute now contains the purified DNA.

Influenza A H11N9 (A/mallard/Alberta/294/1977) cDNA Clone Sequence and Quality Control

Sequence:

1        AAGCTTGCCG CCACCATGAA GAAGGCTCTG CTGTTTGCTG CCATCATCAT CTGTATCCAG
61       GCTGATGAGA TTTGTATTGG CTACCTGAGC AACAACAGCA CAGAGAAGGT GGACACCATC
121      ATTGAGAGCA ATGTGACAGT GACCTCCTCT GTGGAACTGG TGGAGAATGA ACACACAGGC
181      TCCTTCTGTA GCATTGATGG CAAGGCTCCA ATCTCCCTGG GAGACTGTTC CTTTGCTGGC
241      TGGATTCTGG GCAACCCTAT GTGTGATGAC CTGATTGGCA AGACCTCCTG GTCCTACATT
301      GTGGAGAAGC CAAACCCAAT CAATGGCATC TGTTACCCTG GCACCTTGGA GAATGAGGAG
361      GAACTGAGAC TGAAGTTCTC TGGAGTGTTG GAGTTCAGCA AGTTTGAGGC TTTCACCAGC
421      AATGGCTGGG GCTCTGTGAA CTCTGGAGCA GGAGTGACAG CAGCCTGTAA GTTTGGCTCC
481      AGCAACTCCT TCTTCAGGAA TATGGTGTGG CTGATTCACC AGTCTGGCAC CTACCCTGTG
541      ATTAGGAGGA CCTTCAACAA CACCAAGGGC AGGGATGTGC TGATGGTGTG GGGAGTGCAT
601      CACCCTGCCA CCCTGAAAGA ACACCAGGAC CTCTACAAGA AGGACAACTC CTATGTGGCT
661      GTGGGCTCTG AGTCCTACAA CAGGAGGTTC ACACCTGAGA TTAGCACCAG ACCAAAGGTG
721      AATGGACAAG CAGGCAGGAT GACCTTCTAC TGGACCATTG TGAAGCCTGG AGAGGCTATC
781      ACCTTTGAGA GCAATGGAGC CTTCCTGGCT CCAAGATATG CCTTTGAACT GGTGTCCCTG
841      GGCAATGGCA AACTGTTCAG GTCTGACCTG AACATTGAGT CCTGTAGCAC CAAGTGTCAG
901      TCTGAGATTG GAGGCATCAA CACCAACAGG TCCTTCCACT CTGTGCATAG GAACACCATT
961      GGAGACTGTC CAAAATATGT GAATGTGAAG TCCCTGAAAC TGGCTACAGG ACTGAGGAAT
1021     GTGCCTGCCA TTGCTGCCAG GGGACTGTTT GGAGCCATTG CTGGCTTCAT TGAGGGAGGC
1081     TGGCCTGGAC TGATAAATGG CTGGTATGGC TTCCAACACA GGAATGATGA GGGCACAGGC
1141     ATTGCTGCTG ACAAGGAGAG CACCCAGAAG GCTATTGACC AGATTACCAG CAAGGTGAAC
1201     AACATTGTGG ACAGGATGAA CACCAACTTT GAGTCTGTCC AACATGAGTT CTCTGAGATT
1261     GAGGAGAGGA TAAACCAACT TAGCAAACAT GTGGATGACT CTGTGATTGA CATCTGGTCC
1321     TACAATGCCC AACTTCTGGT GCTGTTGGAG AATGAGAAGA CCCTGGACCT CCATGACAGC
1381     AATGTGAGGA ACCTGCATGA GAAGGTGAGG AGGATGCTGA AAGACAATGC CAAGGATGAG
1441     GGCAATGGCT GTTTCACCTT CTACCACAAG TGTGACAATG AGTGTATTGA GAAGGTGAGG
1501     AATGGCACCT ATGACCACAA GGAGTTTGAG GAGGAGAGCA GACTGAACAG ACAGGAGATT
1561     GAGGGAGTGA AACTGGACAG CAATGGCAAT GTCTACAAGA TTCTGAGCAT CTACTCCTGT
1621     ATTGCCTCCT CCCTGGTGCT GGCTGCCATC ATAATGGGCT TCATCTTCTG GGCTTGTAGC
1681     AATGGCTCCT GTAGGTGTAC CATCTGTATT TAAATCTAGA

 

Quality Control:cDNA sequencing results is available upon request.