Influenza A H10N3 (A/duck/Hong Kong/786/1979) Hemagglutinin Gene cDNA Clone / ORF Clone (codon optimized)

Influenza A H10N3 (A/duck/Hong Kong/786/1979) cDNA Clone Datasheet

Influenza A H10N3 (A/duck/Hong Kong/786/1979) cDNA Clone Product Information

• cDNA Size: 1708
• Gene Synonym: HA1, Hemagglutinin
• Source: Influenza Virus

Influenza A H10N3 (A/duck/Hong Kong/786/1979) cDNA Clone Description

• cDNA Description: ORF Clone of Influenza A H10N3 (A/duck/Hong Kong/786/1979) HA DNA.

  This cDNA clone has gone through customized codon optimization in order to obtain high level of protein expression in particular cell lines. Therefore, although the translated amino acid sequence is identical to the amino sequence on Gene Bank, the DNA sequence is different from that on Gene Bank.

• Vector: Vector 3. Restriction site: Hind III + XbaI

Influenza A H10N3 (A/duck/Hong Kong/786/1979) cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

Influenza A H10N3 (A/duck/Hong Kong/786/1979) cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O . Ensure the filter paper is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95℃ for 15-30 min.
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the scattered filter paper from the elute. The elute now contains the purified DNA.

Influenza A H10N3 (A/duck/Hong Kong/786/1979) cDNA Clone Sequence and Quality Control

Sequence:

1        AAGCTTGCCG CCACCATGTA TAAGATTGCT GTGATTATTG CCCTGCTGGG AGCAGTGAGG
61       GGACTGGACA AGATTTGTCT GGGACACCAT GCTGTGGCTA ATGGCACCAT TGTGAAGACC
121      CTGACCAATG AACAGGAGGA GGTGACCAAT GCCACAGAGA CAGTGGAGAG CACCTCCCTG
181      AACAAACTGT GTATGAAGGG CAGGTCCTAC AAGGACCTGG GCAACTGTCA CCCAATTGGG
241      ATGCTGATTG GCACACCTGC CTGTGACCTG CACCTGACAG GCACCTGGGA CACCCTGATT
301      GAGAGGGAGA ATGCCATTGC CTACTGTTAC CCTGGAGCCA CAGTGAATGA GGAGGCTCTG
361      AGACAGAAGA TAATGGAGTC TGGAGGCATC AGCAAGATTA GCACAGGCTT CACCTATGGC
421      TCCAGCATCA ACTCTGCTGG CACCACCAAG GCTTGTATGA GGAATGGAGG CAACTCCTTC
481      TATGCTGAAC TGAAATGGCT GGTGAGCAAG AGCAAGGGAC AAAACTTTCC ACAGACCACC
541      AACACCTACA GGAACACAGA CACAGCAGAA CACCTGATTA TCTGGGGCAT CCACCACCCA
601      TCCAGCACCC AGGAGAAGAA TGACCTCTAT GGCACCCAGT CCCTGAGCAT CAGCATTGGC
661      TCCAGCACCT ACCAGAACAA CTTTGTGCCT GTGGTGGGAG CCAGACCACA GGTGAATGGA
721      CAATCTGGCA GGATTGACTT CCACTGGACC CTGGTCCAAC CTGGAGACAA CATCACCTTC
781      AGCCACAATG GAGGACTGAT TGCCCCAAGC AGGGTGAGCA AACTGATTGG CAGGGGACTG
841      GGCATCCAGT CTGAGGCAAG CATTGACAAT GGCTGTGAGA GCAAGTGTTT CTGGAGGGGA
901      GGCAGCATCA ACACCAAACT GCCATTCCAG AACCTGAGCC CAAGGACAGT GGGACAATGT
961      CCAAAATATG TGAACAAGAA GTCCCTGATG CTGGCTACAG GGATGAGGAA TGTGCCTGAG
1021     ATAATGCAGG GCAGGGGACT GTTTGGAGCC ATTGCTGGCT TCATTGAGAA TGGCTGGGAG
1081     GGGATGGTGG ATGGCTGGTA TGGCTTCAGA CACCAGAATG CCCAGGGCAC AGGACAAGCA
1141     GCAGACTACA AGAGCACCCA GGCTGCCATT GACCAGATTA CAGGCAAACT GAACAGACTG
1201     ATTGAGAAGA CCAACACAGA GTTTGAGAGC ATTGAGTCTG AGTTCTCTGA GATTGAACAC
1261     CAGATTGGCA ATGTGATAAA CTGGACCAAG GACAGCATCA CAGACATCTG GACCTACCAG
1321     GCTGAACTGC TGGTGGCTAT GGAGAACCAA CACACCATTG ATATGGCTGA CTCTGAGATG
1381     CTGAACCTCT ATGAGAGGGT GAGGAAGCAA CTTAGACAGA ATGCTGAGGA GGATGGCAAG
1441     GGCTGTTTTG AGATTTACCA CACTTGTGAT GACTCCTGTA TGGAGAGCAT CAGGAACAAC
1501     ACCTATGACC ACAGCCAATA CAGGGAGGAG GCTCTGCTGA ACAGACTGAA CATCAACCCT
1561     GTGAAACTGT CCTCTGGCTA CAAGGACATC ATCCTGTGGT TCTCCTTTGG AGCCTCCTGT
1621     TTTGTGCTGC TGGCTGTGGT GATGGGACTG GTGTTCTTCT GTCTGAAAAA TGGCAATATG
1681     AGGTGTACCA TCTGTATTTA AATCTAGA

 

Quality Control:cDNA sequencing results is available upon request.