Influenza A H10N3 (A/duck/Hong Kong/786/1979) Hemagglutinin

Influenza A H10N3 ( A/duck/Hong Kong/786/1979 ) HA Protein

H10N3 ( A/duck/Hong Kong/786/1979 ) HA Protein Product Information

• Synonym: HA
• Protein Construction: A DNA sequence encoding the extracellular domain ( Met 1- Ile 525 ) of the influenza A H10N3 hemagglutinin (A/duck/Hong Kong/786/1979 (H10N3)) (BAF46762.1) (HA1+HA2, uncleaved) was fused with a C-terminal polyhistidine tag
• Source: Influenza A H10N3
• Expression Host: Human Cells

H10N3 ( A/duck/Hong Kong/786/1979 ) HA Protein QC Testing

• Purity: > 95 % as determined by SDS-PAGE
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Asp 18
• Molecular Mass: The secreted recombinant influenza A H10N3 HA ( A/duck/Hong Kong/ 786/1979 (H10N3) ) comprises 519 amino acids and has a predicted molecular mass of 57.8 kDa. As a result of glycosylation, it migrates as an approximately 60-65 kDa band in SDS-PAGE under reducing conditions.
• Formulation: Lyophilized from sterile PBS , pH 7.4
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.

• SDS-PAGE: H10N3 HA protein

H10N3 ( A/duck/Hong Kong/786/1979 ) HA Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Related Influenza Virus Research Tools

• Influenza Virus Research Tools
• Influenza Hemagglutinin & Antibody
• Influenza Hemagglutinin Proteins
• H10N3 Hemagglutinin & Antibody
• A/duck/Hong Kong/786/1979

H10N3 ( A/duck/Hong Kong/786/1979 ) HA Protein Description

Influenza (flu) is a respiratory infection in mammals and birds. This virus is divided into three main types (A, B and C). Influenza A is found in a wide variety of bird and mammal species. Influenza B is largely confined to humans and is an important cause of morbidity. Influenza C infects humans, dogs and pigs, sometimes causing both severe illness and local epidemics. Influenza A is further divided into subtypes based on differences in the membrane proteins hemagglutinin (HA) and neuraminidase (NA). The notation HhNn is used to refer to the subtype comprising the hth discovered HA protein and the nth discovered NA protein. The HA is a trimer with a receptor binding pocket on the globular head of each monomer. Subtypes are further divided into strains. Each genetically distinct virus isolate is usually considered to be a separate strain. 
H10N3 is a subtype of Influenza A. Hemagglutinin (HA) is a single-pass type I  integral membrane glycoprotein from the influenza virus, and comprises over 80% of the envelope proteins present in the virus particle. It is a homotrimer of disulfide-linked HA1-HA2. Binding of HA to sialic acid-containing receptors on the surface of its target cell brings about the attachment of the virus particle to the cell and forms a endosome. Low pH in endosomes induce an irreversible conformational change in HA2, releasing the hydrophobic portion “fusion peptide”. After which, virus penetrates the cell and pours its contents including the RNA genome into the cytoplasm mediated by fusion of the endocytosed virus particle’s own membrane and the endosomal membrane. Hemagglutinin plays a major role in the determination of host range restriction and virulence.

References

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7. CDC – Influenza (Flu): Weekly Report: Influenza Summary Update 20, 2004–2005 Season.