Anti-Granzyme H Antibody (Mouse Monoclonal antibody) General Information
Anti-Granzyme H Antibody
Reacts with: Human
Human Granzyme H
No cross-reactivity in ELISA with
Human Granzyme B
Human cell lysate (293 cell line)
Recombinant Human Granzyme H / GZMH protein (Catalog#10348-H08H)
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human Granzyme H / GZMH (rh Granzyme H / GZMH; Catalog#10348-H08H; NP_219491.1; Met 1-Leu 246). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
Monoclonal Mouse IgG2a Clone #3G10F4
0.2 μm filtered solution in PBS with 5% trehalose
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Anti-Granzyme H Antibody (Mouse Monoclonal antibody) Validated Applications
**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********
Anti-Granzyme H Antibody Alternative Names
Anti-CCP-X Antibody;Anti-CGL-2 Antibody;Anti-CSP-C Antibody;Anti-CTLA1 Antibody;Anti-CTSGL2 Antibody;Anti-GZMH Antibody
Granzyme H Background Information
Granzymes are key components of the immune response that play important roles in eliminating host cells infected by intracellular pathogens. Several granzymes are potent inducers of cell death. A total of eight granzymes (A-G and M) have been identified in the mouse, but only five are known in humans (A, B, H, M and granzyme 3), and granzyme H appears to be specifically human. Human granzyme H is a neutral serine protease that is expressed predominantly in the lymphokine-activated killer (LAK)/natural?killer (NK) compartment of the immune system. In adenovirus-infected cells in which granzyme B (gzmB) and downstream apoptosis pathways are inhibited, granzyme H directly cleaves the adenovirus DNA-binding protein (DBP), a viral component absolutely required for viral DNA replication. This virus demonstrated that gzmH directly induces an important decay in viral DNA replication. Interestingly, gzmH also cleaves the adenovirus 100K assembly protein, a major inhibitor of gzmB, and relieves gzmB inhibition. Granzyme H has a very high amino acid identity (>90%) with many portions of the granzyme B sequence, particularly near the amino terminus of the molecule despite performing a distinct enzymic function.
Meier M., et al.,(1990), Cloning of a gene that encodes a new member of the human cytotoxic cell protease family. Biochemistry 29:4042-4049.Haddad P., et al., (1991), Structure and evolutionary origin of the human granzyme H gene.Int. Immunol. 3:57-66.Klein J.L., et al.,(1990), Characterization of a novel, human cytotoxic lymphocyte-specific serine protease cDNA clone (CSP-C).Tissue Antigens 35:220-228.