Granzyme H / GZMH Antibody

Granzyme H / GZMH Antibody Datasheet

Granzyme H / GZMH Antibody Product Information

• Immunogen: Recombinant Human Granzyme H / GZMH protein (Catalog#10348-H08H)
• Antibody Type: Mouse Monoclonal Antibody ( Mouse mAb Service Platform )
• Clone ID: 3G10F4
• Ig Type: Mouse IgG2a
• Formulation: 0.2 μm filtered solution in PBS with 5% trehalose
• Preparation: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human Granzyme H / GZMH (rh Granzyme H / GZMH; Catalog#10348-H08H; NP_219491.1; Met 1-Leu 246). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.

Granzyme H / GZMH Antibody Usage Guide

• Specificity: Human Granzyme H / GZMH
• No cross-reactivity in ELISA with

  Human Granzyme B

  Human cell lysate (293 cell line)
• Direct ELISA: This antibody can be used at 0.5-1 μg/mL with the appropriate secondary reagents to detect Human Granzyme H. The detection limit for Human Granzyme H is approximately 0.078 ng/well.
• Storage: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
  Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

Granzyme H / GZMH Antibody Background

Granzymes are key components of the immune response that play important roles in eliminating host cells infected by intracellular pathogens. Several granzymes are potent inducers of cell death. A total of eight granzymes (A-G and M) have been identified in the mouse, but only five are known in humans (A, B, H, M and granzyme 3), and granzyme H appears to be specifically human. Human granzyme H is a neutral serine protease that is expressed predominantly in the lymphokine-activated killer (LAK)/natural killer (NK) compartment of the immune system. In adenovirus-infected cells in which granzyme B (gzmB) and downstream apoptosis pathways are inhibited, granzyme H directly cleaves the adenovirus DNA-binding protein (DBP), a viral component absolutely required for viral DNA replication. This virus demonstrated that gzmH directly induces an important decay in viral DNA replication. Interestingly, gzmH also cleaves the adenovirus 100K assembly protein, a major inhibitor of gzmB, and relieves gzmB inhibition. Granzyme H has a very high amino acid identity (>90%) with many portions of the granzyme B sequence, particularly near the amino terminus of the molecule despite performing a distinct enzymic function.

References

1. Joseph A Trapani, 2001, Genome Biology, 2 (12): 3014.1-3014.7.
2. Debra M. MacIvor, et al., 1999, Blood. 3: 963-73.
3. Patrick Haddad, et al., 1991, International Immunology. 1: 57-66.
4. Karin A. Sedelies, et al., 2004, Journal of Biological Chemistry. 25: 26581-7.
5. Felipe andrade, et al., 2007, EMBO J., 26 (8): 2148-2157.