Mouse GZMB Gene cDNA Clone / ORF Clone

GZMB cDNA Clone Datasheet

GZMB cDNA Clone Product Information

• Gene Bank Ref.ID: NM_013542.2
• cDNA Size: 744
• Gene Synonym: GZB; CCP1; Ctla1; Ctla-1; AI553453; CCP-1/C11; Gzmb
• Source: Mouse

GZMB cDNA Clone Description

• cDNA Description: ORF Clone of Mus musculus granzyme B DNA.
• Vector: pGEM-T Vector
• Sequence Description: Identical with the Gene Bank Ref.ID sequence.

GZMB cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

GZMB cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95℃ for 15-30 min.
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

GZMB cDNA Clone Sequence and Quality Control

Sequence:

1        ATGAAGATCC TCCTGCTACT GCTGACCTTG TCTCTGGCCT CCAGGACAAA GGCAGGGGAG
61       ATCATCGGGG GACATGAAGT CAAGCCCCAC TCTCGACCCT ACATGGCCTT ACTTTCGATC
121      AAGGATCAGC AGCCTGAGGC GATATGTGGG GGCTTCCTTA TTCGAGAGGA CTTTGTGCTG
181      ACTGCTGCTC ACTGTGAAGG AAGTATAATA AATGTCACTT TGGGGGCCCA CAACATCAAA
241      GAACAGGAGA AGACCCAGCA AGTCATCCCT ATGGTAAAAT GCATTCCCCA CCCAGACTAT
301      AATCCTAAGA CATTCTCCAA TGACATCATG CTGCTAAAGC TGAAGAGTAA GGCCAAGAGG
361      ACTAGAGCTG TGAGGCCCCT CAACCTGCCC AGGCGCAATG TCAATGTGAA GCCAGGAGAT
421      GTGTGCTATG TGGCTGGTTG GGGAAGGATG GCCCCAATGG GCAAATACTC AAACACGCTA
481      CAAGAGGTTG AGCTGACAGT ACAGAAGGAT CGGGAGTGTG AGTCCTACTT TAAAAATCGT
541      TACAACAAAA CCAATCAGAT ATGTGCGGGG GACCCAAAGA CCAAACGTGC TTCCTTTCGG
601      GGGGATTCTG GAGGCCCGCT TGTGTGTAAA AAAGTGGCTG CAGGCATAGT TTCCTATGGA
661      TATAAGGATG GTTCACCTCC ACGTGCTTTC ACCAAAGTCT CGAGTTTCTT ATCCTGGATA
721      AAGAAAACAA TGAAAAGCAG CTAA

 

Quality Control:cDNA sequencing results is available upon request.