Anti-Granzyme B Antibody (Rabbit Monoclonal antibody) General Information
Anti-Granzyme B Antibody
Reacts with: Human
Human Granzyme B
Has cross-reactivity in ELISA with
Human Granzyme H
Recombinant Human Granzyme B protein (Catalog#10345-H08H)
This antibody was obtained from a rabbit immunized with purified, recombinant Human Granzyme B (rh Granzyme B; Catalog#10345-H08H; NP_004122.1; Met 1-Tyr 247)
Monoclonal Rabbit IgG Clone #2
0.2 μm filtered solution in PBS with 5% trehalose
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Anti-Granzyme B Antibody (Rabbit Monoclonal antibody) Validated Applications
**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********
Anti-Granzyme B Antibody (Rabbit Monoclonal antibody) Images
Immunochemical staining of human Granzyme B in human tonsil with rabbit monoclonal antibody (1:1000, formalin-fixed paraffin embedded sections).
Immunochemical staining of human Granzyme B in human spleen with rabbit monoclonal antibody (1:1000, formalin-fixed paraffin embedded sections).
Immunochemical staining of human Granzyme B in human lymph node with rabbit monoclonal antibody (1:1000, formalin-fixed paraffin embedded sections).
Anti-Granzyme B Antibody Alternative Names
Anti-CCPI Antibody;Anti-CGL-1 Antibody;Anti-CGL1 Antibody;Anti-CSP-B Antibody;Anti-CSPB Antibody;Anti-CTLA1 Antibody;Anti-CTSGL1 Antibody;Anti-HLP Antibody;Anti-SECT Antibody
Granzyme B Background Information
Granzyme B, also known as GZMB, is the most prominent member of the granzyme family of cell death-inducing serine proteases expressed in the granules of cytotoxic T lymphocytes (CTLs) and NK cells. Granzyme B enters the target cells depending on another membrane-binding granule protein, perforin, results in the activation of effector caspases and mitochondrial depolarization through caspase-dependent and -independent pathways, and consequently induces rapid cell apoptosis. Over 30 substrates of GZMB have been identified including the key substrate caspase-3, ICAD and Bid. GZMB is suggested to protect the host by lysing cells bearing on their surface 'nonself' antigens such as bacterial and viral infected-cells and tumor cells, and accordingly plays an essential role in immunosurveillance.
granzyme B (granzyme 2, cytotoxic T-lymphocyte-associated serine esterase 1)
Schmid J., et al.,(1987), Induction of mRNA for a serine protease and a beta-thromboglobulin-like protein in mitogen-stimulated human leukocytes. J. Immunol. 139:250-256.Caputo A., et al., (1988), Structure and differential mechanisms of regulation of expression of a serine esterase gene in activated human T lymphocytes.J. Biol. Chem. 263:6363-6369.Trapani J.A., et al.,(1988), Molecular cloning of an inducible serine esterase gene from human cytotoxic lymphocytes.Proc. Natl. Acad. Sci. U.S.A. 85:6924-6928.