Anti-Granzyme B Antibody

Cat: 10345-RP04
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All Granzyme B Reagents
Anti-Granzyme B Antibody (Rabbit Polyclonal antibody) General Information
Product name
Anti-Granzyme B Antibody
Validated applications
Species reactivity
Reacts with: Human
Human Granzyme B
Recombinant Human Granzyme B protein (Catalog#10345-H08H)
Produced in rabbits immunized with purified, recombinant Human Granzyme B (rh Granzyme B; Catalog#10345-H08H; NP_004122.1; Met 1-Tyr 247). Granzyme B specific IgG was purified by human Granzyme B affinity chromatography.
Polyclonal Rabbit IgG
Protein A & Antigen Affinity
0.2 μm filtered solution in PBS with 5% trehalose
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Anti-Granzyme B Antibody (Rabbit Polyclonal antibody) Validated Applications
Application Dilution Notes
WB 1:500-1:1000  
ELISA 1:5000-1:10000  
IHC-P 1:2500-1:10000  
IP 0.2-1 μL/mg of lysate  

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-Granzyme B Antibody (Rabbit Polyclonal antibody) Images
Human Granzyme B/GZMB Immunohistochemistry(IHC) 4093
Immunochemical staining of human Granzyme B in human lymph node with rabbit polyclonal antibody (1:5000, formalin-fixed paraffin embedded sections).
Human Granzyme B/GZMB Immunoprecipitation(IP) 7976

GranzymeB was immunoprecipitated using:

Lane A:0.5 mg NIH-3T3 Whole Cell Lysate

0.5 µL anti-GranzymeB rabbit polyclonal antibody and 15 μl of 50 % Protein G agarose.

Primary antibody:

Anti-GranzymeB rabbit polyclonal antibody,at 1:1000 dilution

Secondary antibody:

Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution

Developed using the odssey technique.

Performed under reducing conditions.

Predicted band size: 28 kDa

Observed band size: 28 kDa

Human Granzyme B/GZMB Western blot (WB) 13194

Anti-GranzymeB rabbit polyclonal antibody at 1:500 dilution

Lane A: NIH/3T3 Whole Cell Lysate

Lysates/proteins at 30 μg per lane.


Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:28 kDa

Observed band size:23 kDa

Anti-Granzyme B Antibody Alternative Names
Anti-CCPI Antibody;Anti-CGL-1 Antibody;Anti-CGL1 Antibody;Anti-CSP-B Antibody;Anti-CSPB Antibody;Anti-CTLA1 Antibody;Anti-CTSGL1 Antibody;Anti-HLP Antibody;Anti-SECT Antibody
Granzyme B Background Information

Granzyme B, also known as GZMB, is the most prominent member of the granzyme family of cell death-inducing serine proteases expressed in the granules of cytotoxic T lymphocytes (CTLs) and NK cells. Granzyme B enters the target cells depending on another membrane-binding granule protein, perforin, results in the activation of effector caspases and mitochondrial depolarization through caspase-dependent and -independent pathways, and consequently induces rapid cell apoptosis. Over 30 substrates of GZMB have been identified including the key substrate caspase-3, ICAD and Bid. GZMB is suggested to protect the host by lysing cells bearing on their surface 'nonself' antigens such as bacterial and viral infected-cells and tumor cells, and accordingly plays an essential role in immunosurveillance.

Full Name
granzyme B (granzyme 2, cytotoxic T-lymphocyte-associated serine esterase 1)
  • Schmid J., et al.,(1987), Induction of mRNA for a serine protease and a beta-thromboglobulin-like protein in mitogen-stimulated human leukocytes. J. Immunol. 139:250-256.
  • Caputo A., et al., (1988), Structure and differential mechanisms of regulation of expression of a serine esterase gene in activated human T lymphocytes.J. Biol. Chem. 263:6363-6369.
  • Trapani J.A., et al.,(1988), Molecular cloning of an inducible serine esterase gene from human cytotoxic lymphocytes.Proc. Natl. Acad. Sci. U.S.A. 85:6924-6928.
  • Product Description Host Clonality Application Catalog# (PDF)
    Anti-Granzyme B Antibody Mouse Monoclonal ELISA,IHC-P 10345-MM01
    Anti-Granzyme B Antibody Rabbit Polyclonal ELISA 10345-RP03
    Anti-Granzyme B Antibody Rabbit Polyclonal WB,ELISA 10345-T48
    Anti-Granzyme B Antibody Rabbit Monoclonal ELISA,IHC-P 10345-R002
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