|IP||0.2-1 μL/mg of lysate|
**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********
GranzymeB was immunoprecipitated using:
Lane A:0.5 mg NIH-3T3 Whole Cell Lysate0.5 µL anti-GranzymeB rabbit polyclonal antibody and 15 μl of 50 % Protein G agarose.Primary antibody:
Anti-GranzymeB rabbit polyclonal antibody,at 1:1000 dilutionSecondary antibody:
Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.
Performed under reducing conditions.Predicted band size: 28 kDa
Observed band size: 28 kDa
Anti-GranzymeB rabbit polyclonal antibody at 1:500 dilution
Lane A: NIH/3T3 Whole Cell LysateLysates/proteins at 30 μg per lane.
Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.
Performed under reducing conditions.Predicted band size:28 kDa
Observed band size:23 kDa
Granzyme B, also known as GZMB, is the most prominent member of the granzyme family of cell death-inducing serine proteases expressed in the granules of cytotoxic T lymphocytes (CTLs) and NK cells. Granzyme B enters the target cells depending on another membrane-binding granule protein, perforin, results in the activation of effector caspases and mitochondrial depolarization through caspase-dependent and -independent pathways, and consequently induces rapid cell apoptosis. Over 30 substrates of GZMB have been identified including the key substrate caspase-3, ICAD and Bid. GZMB is suggested to protect the host by lysing cells bearing on their surface 'nonself' antigens such as bacterial and viral infected-cells and tumor cells, and accordingly plays an essential role in immunosurveillance.
|Product Description||Host||Clonality||Application||Catalog# (PDF)|
|Anti-Granzyme B Antibody||Mouse||Monoclonal||ELISA,IHC-P||10345-MM01|
|Anti-Granzyme B Antibody||Rabbit||Polyclonal||ELISA||10345-RP03|
|Anti-Granzyme B Antibody||Rabbit||Polyclonal||WB,ELISA||10345-T48|
|Anti-Granzyme B Antibody||Rabbit||Monoclonal||ELISA,IHC-P||10345-R002|