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Granzyme B / GZMB Antibody, Mouse MAb

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Human GranzymeB/GZMB Antibody Product Information
Immunogen:Recombinant Human Granzyme B protein (Catalog#10345-H08H)
Clone ID:2F5F2D10
Ig Type:Mouse IgG2b
Concentration:
Endotoxin:
Formulation:0.2 μm filtered solution in PBS with 5% trehalose
Preparation:This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human Granzyme B (rhGranzyme B; Catalog#10345-H08H; NP_004122.1; Met 1-Tyr 247). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
Human GranzymeB/GZMB Antibody Usage Guide
Specificity:Human Granzyme B / GZMB
Application:ELISA, IHC-P

ELISA: 0.5-1 μg/mL

This antibody can be used at 0.5-1 μg/mL with the appropriate secondary reagents to detect Human Granzyme B. The detection limit for Human Granzyme B is approximately 10 ng/well.

IHC-P: 10-30 μg/mL

Storage:This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Human GranzymeB/GZMB Antibody IHC Application Image
Granzyme B / GZMB Antibody, Mouse MAb, Immunohistochemistry
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Caption:
Immunochemical staining of human Granzyme B in human lymph node with mouse monoclonal antibody (15µg/mL, formalin-fixed paraffin embedded sections).
Granzyme B / GZMB Antibody, Mouse MAb, Immunohistochemistry
[Click to enlarge image]
Caption:
Immunochemical staining of human Granzyme B in human spleen with mouse monoclonal antibody (15µg/mL, formalin-fixed paraffin embedded sections).
Granzyme B / GZMB Antibody, Mouse MAb, Immunohistochemistry
[Click to enlarge image]
Caption:
Immunochemical staining of human Granzyme B in human tonsil with mouse monoclonal antibody (15µg/mL, formalin-fixed paraffin embedded sections).
Other GranzymeB/GZMB Antibody Products
Granzyme B/GZMB Background

Granzyme B, also known as GZMB, is the most prominent member of the granzyme family of cell death-inducing serine proteases expressed in the granules of cytotoxic T lymphocytes (CTLs) and NK cells. Granzyme B enters the target cells depending on another membrane-binding granule protein, perforin, results in the activation of effector caspases and mitochondrial depolarization through caspase-dependent and -independent pathways, and consequently induces rapid cell apoptosis. Over 30 substrates of GZMB have been identified including the key substrate caspase-3, ICAD and Bid. GZMB is suggested to protect the host by lysing cells bearing on their surface 'nonself' antigens such as bacterial and viral infected-cells and tumor cells, and accordingly plays an essential role in immunosurveillance.

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Catalog: 10345-MM01-50
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All information of our products is subject to change without notice. Please refer to COA enclosed in shipped package for the newest information.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"