Anti-Granzyme B Antibody (Mouse Monoclonal antibody) General Information
Anti-Granzyme B Antibody
Reacts with: Human
Human Granzyme B
Recombinant Human Granzyme B protein (Catalog#10345-H08H)
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human Granzyme B (rhGranzyme B; Catalog#10345-H08H; NP_004122.1; Met 1-Tyr 247). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
Monoclonal Mouse IgG2b Clone #2F5F2D10
0.2 μm filtered solution in PBS
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Anti-Granzyme B Antibody (Mouse Monoclonal antibody) Validated Applications
**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********
Anti-Granzyme B Antibody (Mouse Monoclonal antibody) Images
Immunochemical staining of human Granzyme B in human tonsil with mouse monoclonal antibody (1:60, formalin-fixed paraffin embedded sections).
Immunochemical staining of human Granzyme B in human spleen with mouse monoclonal antibody (1:60, formalin-fixed paraffin embedded sections).
Immunochemical staining of human Granzyme B in human lymph node with mouse monoclonal antibody (1:60, formalin-fixed paraffin embedded sections).
Anti-Granzyme B Antibody Alternative Names
Anti-CCPI Antibody;Anti-CGL-1 Antibody;Anti-CGL1 Antibody;Anti-CSP-B Antibody;Anti-CSPB Antibody;Anti-CTLA1 Antibody;Anti-CTSGL1 Antibody;Anti-HLP Antibody;Anti-SECT Antibody
Granzyme B Background Information
Granzyme B, also known as GZMB, is the most prominent member of the granzyme family of cell death-inducing serine proteases expressed in the granules of cytotoxic T lymphocytes (CTLs) and NK cells. Granzyme B enters the target cells depending on another membrane-binding granule protein, perforin, results in the activation of effector caspases and mitochondrial depolarization through caspase-dependent and -independent pathways, and consequently induces rapid cell apoptosis. Over 30 substrates of GZMB have been identified including the key substrate caspase-3, ICAD and Bid. GZMB is suggested to protect the host by lysing cells bearing on their surface 'nonself' antigens such as bacterial and viral infected-cells and tumor cells, and accordingly plays an essential role in immunosurveillance.
granzyme B (granzyme 2, cytotoxic T-lymphocyte-associated serine esterase 1)
Schmid J., et al.,(1987), Induction of mRNA for a serine protease and a beta-thromboglobulin-like protein in mitogen-stimulated human leukocytes. J. Immunol. 139:250-256.Caputo A., et al., (1988), Structure and differential mechanisms of regulation of expression of a serine esterase gene in activated human T lymphocytes.J. Biol. Chem. 263:6363-6369.Trapani J.A., et al.,(1988), Molecular cloning of an inducible serine esterase gene from human cytotoxic lymphocytes.Proc. Natl. Acad. Sci. U.S.A. 85:6924-6928.