Granulin / Progranulin / GRN Antibody

Granulin / Progranulin / GRN Antibody Datasheet

Granulin / Progranulin / GRN Antibody Product Information

• Immunogen: Recombinant Mouse Granulin / Progranulin / GRN protein (Catalog#50396-M08H)
• Antibody Type: Rabbit Monoclonal Antibody ( Rabbit mAb Service Platform )
• Clone ID: 017
• Ig Type: Rabbit IgG
• Formulation: 0.2 μm filtered solution in PBS with 5% trehalose
• Preparation: This antibody was obtained from a rabbit immunized with purified, recombinant Mouse Granulin / Progranulin / GRN (rM Granulin / Progranulin / GRN; Catalog#50396-M08H; NP_032201.2; Met 1-Leu 589).

Granulin / Progranulin / GRN Antibody Usage Guide

• Specificity: Mouse Granulin / Progranulin / GRN
• Has cross-reactivity in ELISA with
  

  Human GRN

• Direct ELISA: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Mouse GRN. The detection limit for Mouse GRN is approximately 0.00975 ng/well.
• Storage: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
  Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

Granulin / Progranulin / GRN Antibody Background

Granulin (GRN), also known as PC cell derived growth factor (PCDGF), is a ubiquitously expressed 88 kDa secreted, glycosylated protein which contains seven tandem repeats (55-57 aa consensus) of a highly conserved 12-cysteine granulin/epithelin motif. Cleavage of the signal peptide produces mature granulin which can be further cleaved into a variety of active, 6 kDa peptides. These smaller cleavage products are named granulin A, granulin B, granulin C, etc. Both the peptides and intact granulin protein regulate cell growth. Different members of the granulin protein family may act as inhibitors, stimulators, or have dual actions on cell growth, and thus perform important functions in normal development, wound healing, and tumorigenesis.Defects in GRN are the cause of ubiquitin-positive frontotemporal dementia (UP-FTD), an autosomal dominant neurodegenerative.

References

1. Bateman, A.et al.,1991,Biochem.Biophys.Res.Commun.173 (3):1161-8.
2. Hoque, M. et al.,2003, Mol. Cell. Biol.23 (5): 1688-702.
3. Hoque, M. et al.,2005, J Biol Chem.280 (14):13648-57.
4. Zhou, Ying. et al.,2008,Cell. Signal.20 (5): 942-8.