Anti-Granulin Antibody

Cat: 10826-MM02
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Anti-Granulin Antibody (Mouse Monoclonal antibody) General Information
Product name
Anti-Granulin Antibody
Validated applications
ELISA,ELISA(Det),ICC/IF,IF
Species reactivity
Reacts with: Human
Specificity
Human Granulin
Immunogen
Recombinant Human Granulin protein (Catalog#10826-H08H)
Preparation
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human Granulin / GRN (rhGRN; Catalog#10826-H08H; Met 1-Leu 593; NP_002078.1). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
Source
Monoclonal Mouse IgG1 Clone #2F3G11
Purification
Protein A
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Anti-Granulin Antibody (Mouse Monoclonal antibody) Validated Applications
Application Dilution Notes
ELISA 1:1000-1:2000  
ICC-IF 1:20-1:100  
ELISA(Det) 1:1000-1:10000 This antibody will detect Human Progranulin / Granulin in ELISA pair set (Catalog: # SEKA10826). In a sandwich ELISA, it can be used as detection antibody when paired with (Catalog: # 10826-MM10).

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-Granulin Antibody (Mouse Monoclonal antibody) Images
Human Progranulin/Granulin Immunofluorescence(IF) 2919
Immunofluorescence staining of Human GRN in Hela cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with mouse anti-Human GRN monoclonal antibody (1:60). Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-mouse IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.
Anti-Granulin Antibody Alternative Names
Anti-CLN11 Antibody;Anti-GEP Antibody;Anti-GP88 Antibody;Anti-Granulin Antibody;Anti-PCDGF Antibody;Anti-PEPI Antibody;Anti-PGRN Antibody;Anti-Progranulin Antibody
Granulin Background Information

&Granulins are a family of secreted, glycosylated peptides that are cleaved from a single precursor protein with 7.5 repeats of a highly conserved 12-cysteine granulin/epithelin motif. The precursor protein, progranulin, is also called proepithelin and PC cell-derived growth factor. Cleavage of the signal peptide produces mature granulin which can be further cleaved into a variety of active, 6 kDa peptides. These smaller cleavage products are named granulin A, granulin B, granulin C, etc. Epithelins 1 and 2 are synonymous with granulins A and B, respectively. Both the peptides and intact granulin protein regulate cell growth. However, different members of the granulin protein family may act as inhibitors, stimulators, or have dual actions on cell growth. Granulin family members are important in normal development, wound healing, and tumorigenesis. Granulins have possible cytokine-like activity. They may play a role in inflammation, wound repair, and tissue remodeling. Granulin-4 promotes proliferation of the epithelial cell line A431 in culture while granulin-3 acts as an antagonist to granulin-4, inhibiting the growth. Granulin expression inhibited Tat transactivation, and tethering experiments showed that this effect was due, at least in part, to a direct action on cyclin T1 in the absence of Tat.

Full Name
granulin
References
  • Hoque M, et al. (2003) The growth factor granulin interacts with cyclin T1 and modulates P-TEFb-dependent transcription. Mol Cell Biol. 23(5): 1688-702.
  • Bateman A, et al. (1990) Granulins, a novel class of peptide from leukocytes. Biochem Biophys Res Commun. 173(3): 1161-8.
  • Trinh DP, et al. (1999) Epithelin/granulin growth factors: extracellular cofactors for HIV-1 and HIV-2 Tat proteins. Biochem Biophys Res Commun. 256(2): 299-306.
  • Product Description Host Clonality Application Catalog# (PDF)
    Anti-Granulin Antibody Rabbit Polyclonal ELISA,IHC-P 50396-RP02
    Anti-Granulin Antibody Rabbit Monoclonal ELISA(Det) 50396-R019
    Anti-Granulin Antibody Rabbit Monoclonal ELISA 50396-R017
    Anti-Granulin Antibody Rabbit Monoclonal ELISA,ELISA(Cap) 50396-R034
    Anti-Granulin Antibody Rabbit Polyclonal ELISA 50396-RP01
    Anti-Granulin Antibody Rabbit Polyclonal ELISA,IHC-P 10826-RP04
    Anti-Granulin Antibody Rabbit Monoclonal ELISA 10826-R007
    Anti-Granulin Antibody Mouse Monoclonal ELISA(Cap) 10826-MM10
    Anti-Granulin Antibody Rabbit Polyclonal WB,ELISA 10826-RP03
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