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Granulin / GRN / Progranulin Antibody, Mouse MAb

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Human GRN Antibody Product Information
Immunogen:Recombinant Human Granulin protein (Catalog#10826-H08H)
Clone ID:2F3G11
Ig Type:Mouse IgG1
Formulation:0.2 μm filtered solution in PBS with 5% trehalose
Preparation:This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human Granulin / GRN (rhGRN; Catalog#10826-H08H; Met 1-Leu 593; NP_002078.1). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
Human GRN Antibody Usage Guide
Specificity:Human Granulin / GRN
No cross-reactivity with Human cell lysate (293 cell line) in WB and ELISA.
Application:ELISA, ICC/IF, IF

ELISA: 0.5-1 μg/mL

This antibody can be used at 0.5-1 μg/mL with the appropriate secondary reagents to detect GRN. The detection limit for GRN is approximately 0.16 ng/well.

ICC/IF: 10-25 μg/mL

Storage:This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Human GRN Antibody IF Application Image
Granulin / GRN / Progranulin Antibody, Mouse MAb, Immunofluorescence
[Click to enlarge image]
Immunofluorescence staining of Human GRN in Hela cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with mouse anti-Human GRN monoclonal antibody (15 µg/ml). Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-mouse IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.
Other GRN Antibody Products
Progranulin/Granulin Background

&Granulins are a family of secreted, glycosylated peptides that are cleaved from a single precursor protein with 7.5 repeats of a highly conserved 12-cysteine granulin/epithelin motif. The precursor protein, progranulin, is also called proepithelin and PC cell-derived growth factor. Cleavage of the signal peptide produces mature granulin which can be further cleaved into a variety of active, 6 kDa peptides. These smaller cleavage products are named granulin A, granulin B, granulin C, etc. Epithelins 1 and 2 are synonymous with granulins A and B, respectively. Both the peptides and intact granulin protein regulate cell growth. However, different members of the granulin protein family may act as inhibitors, stimulators, or have dual actions on cell growth. Granulin family members are important in normal development, wound healing, and tumorigenesis. Granulins have possible cytokine-like activity. They may play a role in inflammation, wound repair, and tissue remodeling. Granulin-4 promotes proliferation of the epithelial cell line A431 in culture while granulin-3 acts as an antagonist to granulin-4, inhibiting the growth. Granulin expression inhibited Tat transactivation, and tethering experiments showed that this effect was due, at least in part, to a direct action on cyclin T1 in the absence of Tat.

Human Progranulin/Granulin References
  • Hoque M, et al. (2003) The growth factor granulin interacts with cyclin T1 and modulates P-TEFb-dependent transcription. Mol Cell Biol. 23(5): 1688-702.
  • Bateman A, et al. (1990) Granulins, a novel class of peptide from leukocytes. Biochem Biophys Res Commun. 173(3): 1161-8.
  • Trinh DP, et al. (1999) Epithelin/granulin growth factors: extracellular cofactors for HIV-1 and HIV-2 Tat proteins. Biochem Biophys Res Commun. 256(2): 299-306.
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    Catalog: 10826-MM02-50
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