Gene Summary: This gene product, grancalcin, is a calcium-binding protein abundant in neutrophils and macrophages. Grancalcin belongs to the penta-EF-hand subfamily of proteins which includes sorcin, calpain, and ALG-2. Grancalcin localization is dependent upon calcium and magnesium. In the absence of divalent cation, grancalcin localizes to the cytosolic fraction; with magnesium alone, it partitions with the granule fraction; and in the presence of magnesium and calcium, it associates with both the granule and membrane fractions, suggesting a role for grancalcin in granule-membrane fusion and degranulation. [provided by RefSeq, Jul 2008]General information above from NCBI
Subunit structure: Homodimer. Interacts with SRI and LCP1.
Subcellular location: Cytoplasm. Cytoplasmic granule membrane; Peripheral membrane protein; Cytoplasmic side. Note=Primarily cytosolic in the absence of calcium or magnesium ions. Relocates to granules and other membranes in response to elevated calcium and magnesium levels.
Tissue specificity: Detected in neutrophils and macrophages (at protein level). Highly expressed in bone marrow.
Induction: Up-regulated in the majority of hepatitis C virus- associated hepatocellular carcinoma.
Post-translational: N-glycosylated. The secreted form is modified by hybrid or complex type oligosaccharide chains (By similarity).
Sequence similarity: Contains 4 EF-hand domains.
General information above from UniProt
Liu F, et al. (2004) Characterization of murine grancalcin specifically expressed in leukocytes and its possible role in host defense against bacterial infection. Biosci Biotechnol Biochem. 68(4): 894-902.
Lollike K, et al. (2001) Biochemical characterization of the penta-EF-hand protein grancalcin and identification of L-plastin as a binding partner. J Biol Chem. 276(21): 17762-9.
Jia J, et al. (2000) Crystal structure of human grancalcin, a member of the penta-EF-hand protein family. J Mol Biol. 300(5): 1271-81.
Teahan CG, et al. (1992) Isolation and characterization of grancalcin, a novel 28 kDa EF-hand calcium-binding protein from human neutrophils. Biochem J. 286 (Pt 2): 549-54.