GPC5 / Glypican-5 Protein (His Tag)

Glypican-5 / GPC5 Protein Datasheet

Glypican-5 / GPC5 Protein Product Information

• Synonym: GPC5, bA93M14.1, glypican proteoglycan 5, glypican-5
• Protein Construction: A DNA sequence encoding the human GPC5 ( NP_004457.1 ) ( Met 1 - Thr 554 ) was fused with a polyhistidine tag at the C-terminus
• Source: Human
• Expression Host: Baculovirus-Insect cells

Glypican-5 / GPC5 Protein QC Testing

• Purity: > 92 % as determined by SDS-PAGE
• Bio-activity: Measured by its binding ability in a functional ELISA
  Immobilized human GPC5 at 5 μg/ml ( 100 μl/well ) can bind human bFGF with a linear ranger of 0.156 - 2.5 ng/ml
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Glu 25
• Molecular Mass: The secreted recombinant human GPC5 consists of 540 amino acids and predicts a molecular mass of 60.5 kDa as estimated in SDS-PAGE under reducing conditions
• Formulation: Lyophilized from sterile 50mM Tris ,100mM NaCl , pH 8.0
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: GPC5 protein

Glypican-5 / GPC5 Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Glypican-5 / GPC5 Protein Description

Glypican-5, also known as GPC5, is a cell membrane protein which belongs to the glypican family. The glypicans compose a family of glycosylphosphatidylinositol-anchored heparan sulfate proteoglycans that may play a role in the control of cell division and growth regulation. So far, six members ( Glypican-1 / GPC1, Glypican-2 / GPC2, Glypican-3 / GPC3, Glypican-4 / GPC4, Glypican-5 / GPC5, Glypican-6 / GPC6 ) of this family are known in vertebrates. In adult, Glypican-5 / GPC5 is primarily expressed in the brain. It is also detected in fetal brain, lung and liver. Glypican-5 / GPC5 enhances the intracellular signaling of FGF2 and HGF. It alters the cellular distribution of FGF2. The properties of Glypican-5 / GPC5 make it an attractive target for therapeutic intervention in rhabdomyosarcomas and other tumors that amplify and/or overexpress its gene. Glypican-5 / GPC5 is over-expressed in lymphoma cell lines that had shown amplification. It is a likely target for amplification, and that over-expression of GPC5 may contribute to development and/or progression of lymphomas and other tumors.

References

1. Paine-Saunders, S. et al., 1999, Genomics. 57 (3): 455-8.
2. Veugelers, M. et al., 2001, Matrix Biol. 20 (5-6): 375-85.
3. Maheshwari, M. et al., 2002, BMC Genomics. 3 (1): 30.
4. Yu,W. et al., 2003, J Hum Genet. 48 (6): 331-5.
5. Williamson, D. et al., 2007, Cancer Res. 67 (1): 57-65.