Mouse GPC3 Gene cDNA Clone / ORF Clone

GPC3 cDNA Clone Datasheet

GPC3 cDNA Clone Product Information

• Gene Bank Ref.ID: NM_016697.3
• cDNA Size: 1740
• Gene Synonym: OCI-5; Gpc3
• Source: Mouse

GPC3 cDNA Clone Description

• cDNA Description: ORF Clone of Mus musculus glypican 3 DNA.
• Vector: pGEM-T Vector
• Sequence Description: Identical with the Gene Bank Ref.ID sequence.

GPC3 cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

GPC3 cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95℃ for 15-30 min.
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

GPC3 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGCCGGGA CCGTGCGCAC CGCGTGCTTG CTGGTGGCGA TGCTGCTAGG CTTGGGCTGC
61       CTGGGACAGG CGCAGCCCCC GCCGCCTCCA GACGCCACCT GTCACCAGGT CCGTTCTTTC
121      TTCCAGAGAC TGCAGCCCGG ACTCAAATGG GTTCCAGAAA CCCCTGTACC AGGATCAGAT
181      TTGCAAGTAT GTCTCCCCAA GGGCCCAACA TGCTGCTCAA GAAAGATGGA AGAAAAATAC
241      CAACTAACAG CACGGCTGAA CATGGAACAA CTGCTCCAGT CTGCGAGTAT GGAACTCAAG
301      TTCTTAATTA TTCAGAATGC TGCGGTTTTC CAAGAGGCCT TTGAAATTGT TGTTCGCCAT
361      GCCAAGAACT ACACCAACGC CATGTTCAAG AATAACTACC CCAGCCTGAC TCCACAAGCT
421      TTTGAGTTTG TCGGTGAATT TTTCACAGAT GTGTCTCTCT ACATCTTGGG TTCTGATATC
481      AACGTGGATG ATATGGTCAA TGAATTGTTC GACAGCCTCT TTCCAGTCAT CTACACCCAG
541      ATGATGAACC CAGGCCTGCC TGAGTCAGTC TTAGACATCA ACGAGTGCCT CCGAGGAGCA
601      AGACGTGACC TGAAAGTATT TGGCAGTTTC CCCAAGCTTA TTATGACCCA GGTTTCCAAG
661      TCACTGCAAG TCACTCGAAT CTTCCTTCAA GCCCTGAATC TCGGAATTGA AGTCATCAAC
721      ACTACCGACC ACCTCAAGTT TAGTAAGGAC TGTGGCCGTA TGCTCACCCG AATGTGGTAT
781      TGCTCTTACT GCCAGGGACT GATGATGGTT AAGCCTTGCG GTGGTTATTG CAATGTGGTC
841      ATGCAAGGCT GTATGGCTGG TGTGGTGGAG ATCGACAAGT ACTGGAGAGA ATACATTCTG
901      TCTCTTGAAG AGCTCGTGAA TGGCATGTAC AGAATCTACG ACATGGAGAA TGTGCTGCTC
961      GGCCTCTTTT CTACCATCCA TGATTCCATC CAGTATGTGC AGAAGAACGG AGGCAAGCTG
1021     ACCACCACCA TTGGCAAGTT GTGTGCCCAC TCCCAGCAAC GCCAATATAG ATCTGCTTAT
1081     TACCCTGAAG ATCTGTTTAT TGACAAGAAG ATATTAAAAG TCGCTCATGT CGAACATGAA
1141     GAAACCTTAT CCAGCCGAAG AAGGGAACTG ATTCAGAAAC TGAAGTCTTT CATCAACTTC
1201     TATAGCGCTT TGCCGGGCTA CATCTGCAGC CATAGCCCCG TGGCCGAAAA TGATACCCTG
1261     TGCTGGAACG GACAAGAACT TGTGGAGAGA TACAGCCAGA AGGCGGCAAG GAACGGGATG
1321     AAGAATCAGT TTAACCTCCA TGAGCTGAAA ATGAAGGGCC CTGAGCCGGT GGTTAGCCAG
1381     ATCATTGACA AACTGAAGCA CATTAACCAG CTCCTGAGAA CCATGTCTGT GCCCAAGGGT
1441     AAAGTTCTGG ATAAAAGCCT GGATGAAGAA GGACTTGAAA GTGGAGACTG CGGTGATGAT
1501     GAAGATGAAT GCATTGGAAG CTCTGGTGAC GGGATGGTGA AAGTGAAGAA TCAACTGCGC
1561     TTCCTTGCAG AACTGGCCTA TGATCTGGAT GTGGACGATG CTCCGGGGAA CAAGCAGCAT
1621     GGAAATCAGA AGGACAACGA GATCACCACC TCTCACAGCG TGGGGAACAT GCCGTCCCCA
1681     CTGAAGATCC TCATCAGTGT GGCCATCTAT GTGGCGTGCT TTTTTTTCCT GGTGCACTGA

 

Quality Control:cDNA sequencing results is available upon request.