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Schistosoma japonicum Glutathione S-transferase / GST Protein
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Schistosoma japonicum Glutathione S-transferase / GST Protein PDF Download

Catalog   Size (Price) Quantity In Stock Operation
11213-HNAE  
YES   
 

Protein Production & Purification Service | Recombinant Cytokines & Growth Factors

Glutathione S-transferase Protein Datasheet

 

Glutathione S-transferase / GST Protein Price Inquiry ( Available Sizes )

Glutathione S-transferase / GST Protein Product Information

Synonym : GST
Protein Construction:

A DNA sequence encoding the Glutathione S-transferase (P08515) (Met1-Lys218) was expressed.

Source: Schistosoma japonicum
Expression Host: E.coli

Glutathione S-transferase / GST Protein QC Testing

Purity: > 95 % as determined by SDS-PAGE SDS-PAGE:
SDS-PAGE

Glutathione S-transferase / GST protein

Endotoxin: Please contact us for more information.
Stability: Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N terminal: Met
Molecular Mass:

The recombinant Glutathione S-transferase (GST) consists of 218 amino acids and predicts a molecular mass of 25.5 KDa. It migrates as an approximately 29 KDa band in SDS-PAGE under reducing conditions.

Formulation: Lyophilized from sterile PBS, pH7.5.
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.

Glutathione S-transferase / GST Protein Usage Guide

Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Glutathione S-transferase / GST Protein Related Products & Topics

Related Areas:

Proteins:

Molecule Species Description //For Detailed Info. and Price------CLICK! Cat. No
Glutathione S-transferase/GST Schistosoma japonicum Glutathione S-transferase/GST Protein, Recombinant, Native 11213-HNAB
Glutathione S-transferase/GST Schistosoma japonicum Glutathione S-transferase/GST Protein, Recombinant, Native 11213-HNAE

Antibodies:

Molecule Application Description //For Detailed Info. and Price------CLICK! Cat. No
Schistosoma japonicum Glutathione S-transferase/GST WB GST Tag Antibody, Mouse MAb 11213-MM01
Schistosoma japonicum Glutathione S-transferase/GST ELISA GST Tag Antibody, Mouse MAb 11213-MM02
Schistosoma japonicum Glutathione S-transferase/GST WB, ELISA GST Antibody, Mouse MAb (HRP conjugated) 11213-MM05
Schistosoma japonicum Glutathione S-transferase/GST WB, ELISA GST Tag Antibody, Rabbit PAb 11213-RP03
Schistosoma japonicum Glutathione S-transferase/GST WB, ELISA GST Tag Antibody, Rabbit PAb (Antigen Affinity Purified) 11213-RP04

Glutathione S-transferase / GST Protein Description

Genetic engineers have used glutathione S-transferase to create the GST gene fusion system. This system is used to purify and detect proteins of interest. In a GST gene fusion system, the GST sequence is incorporated into an expression vector alongside the gene sequence encoding the protein of interest. Induction of protein expression from the vector's promoter results in expression of a fusion protein: the protein of interest fused to the GST protein. This GST-fusion protein can then be purified from cells via its high affinity for glutathione. GST is commonly used to create fusion proteins. The tag has the size of 220 amino acids (roughly 26 KDa), which, compared to other tags like the myc- or the FLAG-tag, is quite big. However, many commercially-available sources of GST-tagged plasmids include a thrombin domain for cleavage of the GST tag during protein purification.

References

  1. Douglas KT . et al., 1987, Adv Enzymol Relat Areas Mol Biol.?59: 103- 67.?
  2. Beckett GJ. et al., 1993, ?Adv Clin Chem. 30: 281-380.
  3. Wilce MC. et al., 1994, ?Biochim Biophys Acta. 1205?(1): 1-18.
  4. Leaver MJ. et al., 1998, ?Marine Environmental Research. 46?(1-5): 71-4.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"