GST Protein, Schistosoma japonicum, Recombinant

Cat: 11213-HNAE
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GST Protein, Schistosoma japonicum, Recombinant Product Information
Purity
> 95 % as determined by SDS-PAGE
Endotoxin
Please contact us for more information.
Activity
Testing in progress
Protein Construction
A DNA sequence encoding the Glutathione S-transferase (P08515) (Met1-Lys218) was expressed.
Accession#
Expressed Host
E. coli
Species
Schistosoma japonicum
Predicted N Terminal
Met
Molecule Mass
The recombinant Glutathione S-transferase (GST) consists of 218 amino acids and predicts a molecular mass of 25.5 KDa. It migrates as an approximately 29 KDa band in SDS-PAGE under reducing conditions.
Formulation
Lyophilized from sterile PBS, pH 7.5
1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
2. Please contact us for any concerns or special requirements.
Please refer to the specific buffer information in the hard copy of CoA.
Shipping
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Stability & Storage
Samples are stable for up to twelve months from date of receipt at -70℃
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
GST Protein, Schistosoma japonicum, Recombinant in SDS-PAGE
Schistosoma japonicum Glutathione S-transferase/GST Protein 9476
GST Background Information

Genetic engineers have used glutathione S-transferase to create the GST gene fusion system. This system is used to purify and detect proteins of interest. In a GST gene fusion system, the GST sequence is incorporated into an expression vector alongside the gene sequence encoding the protein of interest. Induction of protein expression from the vector's promoter results in expression of a fusion protein: the protein of interest fused to the GST protein. This GST-fusion protein can then be purified from cells via its high affinity for glutathione. GST is commonly used to create fusion proteins. The tag has the size of 220 amino acids (roughly 26 KDa), which, compared to other tags like the myc- or the FLAG-tag, is quite big. However, many commercially-available sources of GST-tagged plasmids include a thrombin domain for cleavage of the GST tag during protein purification.

References
  • Douglas KT . et al., 1987, Adv Enzymol Relat Areas Mol Biol.?59: 103- 67.?
  • Beckett GJ. et al., 1993, ?Adv Clin Chem. 30: 281-380.
  • Wilce MC. et al., 1994, ?Biochim Biophys Acta. 1205?(1): 1-18.
  • Leaver MJ. et al., 1998, ?Marine Environmental Research. 46?(1-5): 71-4.

Other GST Proteins

Schistosoma japonicum GST Proteins

Product Name Cat.No.
GST Protein, Schistosoma japonicum, Recombinant 11213-HNAB
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