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Glutathione S-transferase/GST Protein, Antibody, ELISA Kit, cDNA Clone

Expression host: E. coli
  • Slide 1
11213-HNAE-100
11213-HNAE-200
100 µg / $78
200 µg / $108
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Expression host: Baculovirus-Insect Cells
  • Slide 1
11213-HNAB-50
11213-HNAB-100
50 µg / $138
100 µg / $198
Add to Cart

Glutathione S-transferase/GST Related Areas

Glutathione S-transferase/GST Related Pathways

Glutathione S-transferase/GST Related Product

    Glutathione S-transferase/GST Summary & Protein Information

    Glutathione S-transferase/GST Background

    Gene Summary: The MAPEG (Membrane Associated Proteins in Eicosanoid and Glutathione metabolism) family consists of six human proteins, two of which are involved in the production of leukotrienes and prostaglandin E, important mediators of inflammation. Other family members, demonstrating glutathione S-transferase and peroxidase activities, are involved in cellular defense against toxic, carcinogenic, and pharmacologically active electrophilic compounds. This gene encodes a protein that catalyzes the conjugation of glutathione to electrophiles and the reduction of lipid hydroperoxides. This protein is localized to the endoplasmic reticulum and outer mitochondrial membrane where it is thought to protect these membranes from oxidative stress. Several transcript variants, some non-protein coding and some protein coding, have been found for this gene.
    General information above from NCBI

    Genetic engineers have used glutathione S-transferase to create the GST gene fusion system. This system is used to purify and detect proteins of interest. In a GST gene fusion system, the GST sequence is incorporated into an expression vector alongside the gene sequence encoding the protein of interest. Induction of protein expression from the vector's promoter results in expression of a fusion protein: the protein of interest fused to the GST protein. This GST-fusion protein can then be purified from cells via its high affinity for glutathione. GST is commonly used to create fusion proteins. The tag has the size of 220 amino acids (roughly 26 KDa), which, compared to other tags like the myc- or the FLAG-tag, is quite big. However, many commercially-available sources of GST-tagged plasmids include a thrombin domain for cleavage of the GST tag during protein purification.

    Glutathione S-transferase/GST Alternative Name

    GST,Glutathione S-transferase, [human]

    Glutathione S-transferase/GST Related Studies

  • Douglas KT . et al., 1987, Adv Enzymol Relat Areas Mol Biol.?59: 103- 67.?
  • Beckett GJ. et al., 1993, ?Adv Clin Chem. 30: 281-380.
  • Wilce MC. et al., 1994, ?Biochim Biophys Acta. 1205?(1): 1-18.
  • Leaver MJ. et al., 1998, ?Marine Environmental Research. 46?(1-5): 71-4.
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