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GST Tag Antibody, Rabbit PAb

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Schistosoma japonicum GST Antibody Product Information
Immunogen:Recombinant GST protein
Clone ID:
Ig Type:Rabbit IgG
Concentration:
Endotoxin:
Formulation:0.2 μm filtered solution in PBS with 5% trehalose
Preparation:Produced in rabbits immunized with purified, recombinant Glutathione S-transferase (GST). Total IgG was purified by Protein A affinity chromatography.
Schistosoma japonicum GST Antibody Usage Guide
Specificity:GST
Application:WB, ELISA

WB: 1/1000-1/2000

ELISA: 1/2000-1/5000

This antibody can be used at 1/2000-1/5000 dilution with the appropriate secondary reagents to detect GST. The detection limit for GST is approximately 0.16 ng/well.

Storage:This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Other GST Antibody Products
Reactivity: Schistosoma japonicum  
Application: WB  
  • Slide 1
11213-MM01-100
11213-MM01-50
11213-MM01-500
11213-MM01-5
11213-MM01-1
100 µL 
50 µL 
500 µL 
5 mL 
1 mL 
Add to Cart
Reactivity: Schistosoma japonicum  
Application: ELISA  
    11213-MM02-50
    11213-MM02-100
    50 µL 
    100 µL 
    Add to Cart
    Reactivity: Schistosoma japonicum  
    Application: ELISA  
      11213-RP04-50
      11213-RP04-100
      50 µg 
      100 µg 
      Add to Cart
      Glutathione S-transferase/GST Background

      Genetic engineers have used glutathione S-transferase to create the GST gene fusion system. This system is used to purify and detect proteins of interest. In a GST gene fusion system, the GST sequence is incorporated into an expression vector alongside the gene sequence encoding the protein of interest. Induction of protein expression from the vector's promoter results in expression of a fusion protein: the protein of interest fused to the GST protein. This GST-fusion protein can then be purified from cells via its high affinity for glutathione. GST is commonly used to create fusion proteins. The tag has the size of 220 amino acids (roughly 26 KDa), which, compared to other tags like the myc- or the FLAG-tag, is quite big. However, many commercially-available sources of GST-tagged plasmids include a thrombin domain for cleavage of the GST tag during protein purification.

      Schistosoma japonicum Glutathione S-transferase/GST References
      • Douglas KT . et al., 1987, Adv Enzymol Relat Areas Mol Biol.?59: 103- 67.?
      • Beckett GJ. et al., 1993, ?Adv Clin Chem. 30: 281-380.
      • Wilce MC. et al., 1994, ?Biochim Biophys Acta. 1205?(1): 1-18.
      • Leaver MJ. et al., 1998, ?Marine Environmental Research. 46?(1-5): 71-4.
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      Catalog: 11213-RP03-50
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      All information of our products is subject to change without notice. Please refer to COA enclosed in shipped package for the newest information.
      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"