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GST Tag Antibody, Mouse MAb

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Schistosoma japonicum GST Antibody Product Information
Immunogen:E.coli-derived GST
Clone ID:2G10B6
Ig Type:Mouse IgG2a
Concentration:
Endotoxin:
Formulation:0.2 μm filtered solution in PBS with 5% trehalose
Preparation:This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Glutathione S-transferase (GST). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
Other GST Antibody Products
Reactivity: Schistosoma japonicum  
Application: WB  
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11213-MM01-100
11213-MM01-50
11213-MM01-500
11213-MM01-5
11213-MM01-1
100 µL 
50 µL 
500 µL 
5 mL 
1 mL 
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Reactivity: Schistosoma japonicum  
Application: ELISA  WB  
    11213-RP03-50
    11213-RP03-100
    11213-RP03-5
    11213-RP03-500
    11213-RP03-1
    50 µL 
    100 µL 
    5 mL 
    500 µL 
    1 mL 
    Add to Cart
    Reactivity: Schistosoma japonicum  
    Application: ELISA  
      11213-RP04-50
      11213-RP04-100
      50 µg 
      100 µg 
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      Immunochemical staining of human CCNF in human brain with rabbit polyclonal antibody (1 µg/mL, formalin-fixed paraffin embedded sections).
      Glutathione S-transferase/GST Background

      Genetic engineers have used glutathione S-transferase to create the GST gene fusion system. This system is used to purify and detect proteins of interest. In a GST gene fusion system, the GST sequence is incorporated into an expression vector alongside the gene sequence encoding the protein of interest. Induction of protein expression from the vector's promoter results in expression of a fusion protein: the protein of interest fused to the GST protein. This GST-fusion protein can then be purified from cells via its high affinity for glutathione. GST is commonly used to create fusion proteins. The tag has the size of 220 amino acids (roughly 26 KDa), which, compared to other tags like the myc- or the FLAG-tag, is quite big. However, many commercially-available sources of GST-tagged plasmids include a thrombin domain for cleavage of the GST tag during protein purification.

      Schistosoma japonicum Glutathione S-transferase/GST References
      • Douglas KT . et al., 1987, Adv Enzymol Relat Areas Mol Biol.?59: 103- 67.?
      • Beckett GJ. et al., 1993, ?Adv Clin Chem. 30: 281-380.
      • Wilce MC. et al., 1994, ?Biochim Biophys Acta. 1205?(1): 1-18.
      • Leaver MJ. et al., 1998, ?Marine Environmental Research. 46?(1-5): 71-4.
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      Catalog: 11213-MM02-100
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      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"