Anti-GNS Antibody

Cat: 10319-RP03
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All GNS Reagents
Anti-GNS Antibody (Rabbit Polyclonal antibody) General Information
Product name
Anti-GNS Antibody
Validated applications
Species reactivity
Reacts with: Human
Human GNS
Recombinant Human GNS protein (Catalog#10319-H08H)
Produced in rabbits immunized with purified, recombinant Human GNS / G6S (rhGNS; Catalog#10319-H08H; NP_002067.1; Met 1-Leu 552). GNS specific IgG was purified by human GNS affinity chromatography.
Polyclonal Rabbit IgG
Protein A & Antigen Affinity
0.2 μm filtered solution in PBS with 5% trehalose
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Anti-GNS Antibody (Rabbit Polyclonal antibody) Validated Applications
Application Dilution Notes
WB 1:500-1:1000  
ELISA 1:5000-1:10000  
IP 0.1-0.5 μL/mg of lysate  

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-GNS Antibody (Rabbit Polyclonal antibody) Images
Human Glucosamine (N-acetyl)-6-Sulfatase Immunoprecipitation(IP) 7973

GNS was immunoprecipitated using:

Lane A:0.5 mg 293T Whole Cell Lysate

0.5 µL anti-GNS rabbit polyclonal antibody and 15 μl of 50 % Protein G agarose.

Primary antibody:

Anti-GNS rabbit polyclonal antibody,at 1:1000 dilution

Secondary antibody:

Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution

Developed using the odssey technique.

Performed under reducing conditions.

Predicted band size: 100 kDa

Observed band size: 100 kDa

Human Glucosamine (N-acetyl)-6-Sulfatase Western blot (WB) 14048

Anti-GNS rabbit polyclonal antibody at 1:500 dilution

Lane A: 293T Whole Cell Lysate

Lane B: PC3 Whole Cell Lysate

Lysates/proteins at 30 μg per lane.


Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:100 kDa

Observed band size:95 kDa

(We are unsure as to the identity of these extra bands.)

Anti-GNS Antibody Alternative Names
Anti-G6S Antibody
GNS Background Information

Glucosamine (N-acetyl)-6-sulfatase (GNS), also known as G6S, a hydrolase, which is one of the enzymes involved in heparan sulfate catabolism leading to lysosomal storage. GNS is required for the catabolism of the glycosaminoglycans (GAG) including heparin, heparan sulphate, and keratan sulphate through the hydrolysis of 6-sulfate group from the N-acetyl-D-glucosamine 6-sulfate units. Mucopolysaccharidosis type IIID (MPS IIID) is the least common of the four subtypes of Sanfilippo syndrome. It is caused by a deficiency of N-acetylglucosamine-6-sulphatase. A mutation in GNS resulting in MPS IIID indicates the potential utility of molecular diagnosis for this rare condition. As the least common type of the four subtypes of Sanfilippo syndrome, MPS IIID has profound mental deterioration, hyperactivity, and relatively mild somatic manifestations.

Full Name
glucosamine (N-acetyl)-6-sulfatase
  • Fuchs W, et al. (1985) Intralysosomal formation and metabolic fate of N-acetylglucosamine 6-sulfate from keratan sulfate. Eur J Biochem. 151(3): 551-6.
  • Beesley CE, et al. (2003) Sanfilippo syndrome type D: identification of the first mutation in the N-acetylglucosamine-6-sulphatase gene. J Med Genet. 40(3): 192-4.
  • Mok A, et al. (2003) Genomic basis of mucopolysaccharidosis type IIID (MIM 252940) revealed by sequencing of GNS encoding N-acetylglucosamine-6-sulfatase. Genomics. 81(1): 1-5.
  • Elioglu NH, et al. (2009) A novel loss-of-function mutation in the GNS gene causes Sanfilippo syndrome type D. Genet Couns. 20(2): 133-9.
  • Product Description Host Clonality Application Catalog# (PDF)
    Anti-GNS Antibody Mouse Monoclonal ELISA 10319-MM01
    Anti-GNS Antibody Rabbit Monoclonal WB,ELISA 10319-R007
    Anti-GNS Antibody Rabbit Polyclonal ELISA 10319-RP02
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