|IP||0.1-0.5 μL/mg of lysate|
**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********
GNS was immunoprecipitated using:
Lane A:0.5 mg 293T Whole Cell Lysate0.5 µL anti-GNS rabbit polyclonal antibody and 15 μl of 50 % Protein G agarose.Primary antibody:
Anti-GNS rabbit polyclonal antibody,at 1:1000 dilutionSecondary antibody:
Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.
Performed under reducing conditions.Predicted band size: 100 kDa
Observed band size: 100 kDa
Anti-GNS rabbit polyclonal antibody at 1:500 dilution
Lane A: 293T Whole Cell Lysate
Lane B: PC3 Whole Cell LysateLysates/proteins at 30 μg per lane.
Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.
Performed under reducing conditions.Predicted band size:100 kDa
Observed band size:95 kDa
(We are unsure as to the identity of these extra bands.)
Glucosamine (N-acetyl)-6-sulfatase (GNS), also known as G6S, a hydrolase, which is one of the enzymes involved in heparan sulfate catabolism leading to lysosomal storage. GNS is required for the catabolism of the glycosaminoglycans (GAG) including heparin, heparan sulphate, and keratan sulphate through the hydrolysis of 6-sulfate group from the N-acetyl-D-glucosamine 6-sulfate units. Mucopolysaccharidosis type IIID (MPS IIID) is the least common of the four subtypes of Sanfilippo syndrome. It is caused by a deficiency of N-acetylglucosamine-6-sulphatase. A mutation in GNS resulting in MPS IIID indicates the potential utility of molecular diagnosis for this rare condition. As the least common type of the four subtypes of Sanfilippo syndrome, MPS IIID has profound mental deterioration, hyperactivity, and relatively mild somatic manifestations.