Glucokinase (Protein|Antibody|cDNA Clone|ELISA Kit)

All Glucokinase reagents are produced in house and quality controlled, including 4 Glucokinase Antibody, 2 Glucokinase Gene, 1 Glucokinase Protein. All Glucokinase reagents are ready to use.

Recombinant Glucokinase proteins are expressed by E. coli with fusion tags as N-cleavage.

Glucokinaseantibodies are validated with different applications, which are WB, ELISA.

GlucokinasecDNA clones are full length sequence confirmed and expression validated. There are 13 kinds of tags for each Glucokinase of different species, especially GFP tag, OFP tag, FLAG tag and so on. There are three kinds of vectors for choice, cloning vector, expression vector and lentivrial expression vector.

Glucokinase Protein (1)

Species

Glucokinase Protein, Human, Recombinant

11078-HNCE

Expression host: E. coli

Human Glucokinase Protein 9385
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Glucokinase Antibody (4)

Application Clonality
Host

Anti-Glucokinase Antibody

11078-RP01

Application: ELISA

Clonality: PAb

Anti-Glucokinase Antibody

11078-R223

Application: WB,ELISA

Clonality: MAb

Human Glucokinase Western blot (WB) 19423

Anti-Glucokinase Antibody

11078-T16

Application: ELISA

Clonality: PAb

Anti-Glucokinase Antibody

11078-MM13

Application: ELISA

Clonality: MAb

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Glucokinase cDNA Clone (2)

Human
NM_033507.1

Glucokinase belongs to the bacterial glucokinase family. Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. Alternative splicing of this gene results in three tissue-specific forms of glucokinase, one found in pancreatic islet beta cells and two found in liver. The protein localizes to the outer membrane of mitochondria. In contrast to other forms of hexokinase, this enzyme is not inhibited by its product glucose-6-phosphate but remains active while glucose is abundant. Mutations in this gene have been associated with non-insulin dependent diabetes mellitus (NIDDM), maturity onset diabetes of the young, type 2 (MODY2) and persistent hyperinsulinemic hypoglycemia of infancy (PHHI). It can Catalyzes the initial step in utilization of glucose by the beta-cell and liver at physiological glucose concentration. Glucokinase has a high Km for glucose, and so it is effective only when glucose is abundant. The role of GCK is to provide G6P for the synthesis of glycogen. Pancreatic glucokinase plays an important role in modulating insulin secretion. Hepatic glucokinase helps to facilitate the uptake and conversion of glucose by acting as an insulin-sensitive determinant of hepatic glucose usage. It has a pivotal role as glucose sensor of the pancreatic beta-cells. Glucokinase explains the capacity, hexose specificity, affinities, sigmoidicity, and anomeric preference of pancreatic islet glycolysis, and because stimulation of glucose metabolism is a prerequisite of glucose stimulation of insulin release, glucokinase also explains many characteristics of this beta-cell function. Glucokinase of the beta-cell is induced or activated by glucose in contrast to liver glucokinase, which is regulated by insulin. Tissue-specific regulation corresponds with observations that liver and pancreatic beta-cell glucokinase are structurally distinct. Glucokinase could play a glucose-sensor role in hepatocytes as well, and certain forms of diabetes mellitus might be due to glucokinase deficiencies in pancreatic beta-cells, hepatocytes, or both.

Protein
Antibody
Gene
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