Anti-Galectin-1 Antibody

Cat: 10290-MM02
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Anti-Galectin-1 Antibody (Mouse Monoclonal antibody) General Information
Product name
Anti-Galectin-1 Antibody
Validated applications
WB,IP
Species reactivity
Reacts with: Human
Specificity
Human Galectin-1
Immunogen
Recombinant Human Galectin-1 / LGALS1 protein (Catalog#10290-HNAE)
Preparation
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human Galectin-1 / LGALS1 (rh Galectin-1 / LGALS1; Catalog#10290-HNAE; NP_002296.1; Met1-Asp135). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
Source
Monoclonal Clone #02
Purification
Protein A
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Anti-Galectin-1 Antibody (Mouse Monoclonal antibody) Validated Applications
Application Dilution Notes
WB 1:500-1:1000  
IP 0.2-1 μL/mg of lysate  

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-Galectin-1 Antibody (Mouse Monoclonal antibody) Images
Human Galectin-1/LGALS1 Western blot (WB) 13166

Anti-Galectin1 mouse monoclonal antibody at 1:500

Lane A: Hela Whole Cell Lysate

Lane B: A431 Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size: kDa

Observed band size: kDa

Human Galectin-1/LGALS1 Immunoprecipitation(IP) 17655

Galectin1 was immunoprecipitated using:

Lane A:0.5 mg Hela Whole Cell Lysate

0.5 µL anti-Galectin1 mouse monoclonal antibody and 15 μl of 50 % Protein G agarose.

Primary antibody:

Anti-Galectin1 mouse monoclonal antibody,at 1:250 dilution

Secondary antibody:

Dylight 800-labeled antibody to Mouse IgG (H+L), at 1:7500 dilution

Developed using the odssey technique.

Performed under reducing conditions.

Predicted band size: 15 kDa

Observed band size: 15 kDa

Anti-Galectin-1 Antibody Alternative Names
Anti-GAL1 Antibody;Anti-Galectin-1 Antibody;Anti-GBP Antibody
Galectin-1 Background Information

Galectin-1 (Gal-1, GAL1), is a member of the galectins, a family of animal lectins ranging from Caenorhabditis elegans to humans, which is defined by their affinity for beta-galactosides and by significant sequence similarity in the carbohydrate-binding site. It is a homodimer with a subunit molecular mass of 14.5 kDa, which contains six cysteine residues per subunit. The cysteine residues should be in a free state in order to maintain a molecular structure that is capable of showing lectin activity. This endogenous lectin widely expressed at sites of inflammation and tumour growth, has been postulated as an attractive immunosuppressive agent to restore immune cell tolerance and homeostasis in autoimmune and inflammatory settings. On the other hand, galectin-1 contributes to different steps of tumour progression including cell adhesion, migration and tumour-immune escape, suggesting that blockade of galectin-1 might result in therapeutic benefits in cancer. Several potential glycoprotein ligands for galectin-1 have been identified, including lysosome-associated membrane glycoproteins and fibronectin, laminin, as well as T-cell glycoproteins CD43 and CD45. Evidence points to Gal-1 and its ligands as one of the master regulators of such immune responses as T-cell homeostasis and survival, T-cell immune disorders, inflammation and allergies as well as host-pathogen interactions.

Full Name
lectin, galactoside-binding, soluble, 1
References
  • Gaudet AD, et al. (2005) Expression and functions of galectin-1 in sensory and motoneurons. Curr Drug Targets. 6(4): 419-25.
  • Kadoya T, et al. (2006) Structural and functional studies of galectin-1: a novel axonal regeneration-promoting activity for oxidized galectin-1. Curr Drug Targets. 6(4): 375-83.
  • Camby I, et al. (2006) Galectin-1: a small protein with major functions. Glycobiology. 16(11): 137R-157R.
  • Salatino M, et al. (2008) Galectin-1 as a potential therapeutic target in autoimmune disorders and cancer. Expert Opin Biol Ther. 8(1): 45-57.
  • Product Description Host Clonality Application Catalog# (PDF)
    Anti-Galectin-1 Antibody Mouse Monoclonal ELISA 80248-MM06
    Anti-Galectin-1 Antibody Rabbit Monoclonal ELISA 80248-R018
    Anti-Galectin-1 Antibody Rabbit Polyclonal ELISA 80248-RP01
    Anti-Galectin-1 Antibody Rabbit Polyclonal WB,ELISA,IP 80248-T52
    Anti-Galectin-1 Antibody Rabbit Polyclonal ELISA 50100-RP01
    Anti-Galectin-1 Antibody Rabbit Monoclonal WB,IP 10290-R001
    Anti-Galectin-1 Antibody Rabbit Monoclonal ELISA,ELISA(Det) 10290-R008
    Anti-Galectin-1 Antibody Rabbit Monoclonal ELISA(Cap) 10290-R015
    Anti-Galectin-1 Antibody Mouse Monoclonal IHC-P 10290-MM01
    Anti-Galectin-1 Antibody Rabbit Polyclonal WB,ELISA 10290-RP02
    Anti-Galectin-1 Antibody Mouse Monoclonal ELISA 10290-MM04
    Anti-Galectin-1 Antibody Rabbit Polyclonal ELISA 10290-T16
    Anti-Galectin-1 Antibody Rabbit Polyclonal ELISA 10290-RP01
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