Galactolipase / PNLIPRP2 Protein (His Tag)

Pancreatic lipase-related protein 2 Protein Datasheet

Galactolipase / PNLIPRP2 Protein Product Information

• Synonym: PNLIPRP2,PLRP2
• Protein Construction: A DNA sequence encoding the human PNLIPRP2 (P54317)( Met 1- Cys469) was expressed, with a C-terminal polyhistidine tag.
• Source: Human
• Expression Host: Baculovirus-Insect cells

Galactolipase / PNLIPRP2 Protein QC Testing

• Purity: > 95 % as determined by SDS-PAGE
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Lys 18
• Molecular Mass: The secreted recombinant human PNLIPRP2 consists of 462 amino acids and predicts a molecular mass of 51.5 KDa. The apparent molecular mass of the protein is approximately 57 Kda in SDS-PAGE under reducing conditions due to glycosylation.
• Formulation: Lyophilized from sterile 20mM Tris,500mM NaCl, pH 7.4, 10% gly.
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: Galactolipase / PNLIPRP2 protein

Galactolipase / PNLIPRP2 Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Galactolipase / PNLIPRP2 Protein Description

Galactolipase, also known as PNLIPRP2, is a lipase with broad substrate specificity. Galactolipase contributes to milk fat hydrolysis. It hydrolyses milk fat with a lower catalytic efficiency.  The expressed galactolipase shows a lipolytic activity that is, however, only marginally dependent on the presence of colipase. The lipolytic activity of pancreatic extracts and human pancreatic juice on Labrasol is mainly due to the combined action of carboxyl ester hydrolase and galactolipase. Galactolipase also hydrolyzes both phospholipids and galactolipids. It acts preferentially on monoglycerides, phospholipids and galactolipids.

References

1. Andersson EL. et al., 2011, J Lipid Res. 52 (11): 1949-56.
2. Xiao X. et al., 2011, J Biol Chem. 286 (30): 26353-63.
3. Alves BN. et al., 2009, Cell Biochem Funct. 27 (5): 296-308.