Human GZMH Gene cDNA Clone / ORF Clone

GZMH cDNA Clone Datasheet

GZMH cDNA Clone Product Information

• Gene Bank Ref.ID: BC027974
• cDNA Size: 741
• Gene Synonym: CCP-X; CGL-2; CSP-C; CTLA1; CTSGL2; GZMH
• Source: Human

GZMH cDNA Clone Description

• cDNA Description: ORF Clone of Homo sapiens granzyme H (cathepsin G-like 2, protein h-CCPX) DNA.
• Vector: Cloning Vector: pGEM-T Vector

  Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG

• Sequence Description: Identical with the Gene Bank Ref.ID sequence.

GZMH cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

GZMH cDNA Clone Usage Protocol

1. Cut along the circle edge of the spot (arrow points to) to get the filter paper.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to incubation at 95℃ for 15-30 min.
4. At the end of the incubation pulse vortex, or gently tap the tube.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

GZMH cDNA Clone Sequence and Quality Control

Sequence:

1        ATGCAGCCAT TCCTCCTCCT GTTGGCCTTT CTTCTGACCC CTGGGGCTGG GACAGAGGAG
61       ATCATCGGGG GCCATGAGGC CAAGCCCCAC TCCCGCCCCT ACATGGCCTT TGTTCAGTTT
121      CTGCAAGAGA AGAGTCGGAA GAGGTGTGGC GGCATCCTAG TGAGAAAGGA CTTTGTGCTG
181      ACAGCTGCTC ACTGCCAGGG AAGCTCCATA AATGTCACCT TGGGGGCCCA CAATATCAAG
241      GAACAGGAGC GGACCCAGCA GTTTATCCCT GTGAAAAGAC CCATCCCCCA TCCAGCCTAT
301      AATCCTAAGA ACTTCTCCAA CGACATCATG CTACTGCAGC TGGAGAGAAA GGCCAAGTGG
361      ACCACAGCTG TGCGGCCTCT CAGGCTACCT AGCAGCAAGG CCCAGGTGAA GCCAGGGCAG
421      CTGTGCAGTG TGGCTGGCTG GGGTTATGTC TCAATGAGCA CTTTAGCAAC CACACTGCAG
481      GAAGTGTTGC TGACAGTGCA GAAGGACTGC CAGTGTGAAC GTCTCTTCCA TGGCAATTAC
541      AGCAGAGCCA CTGAGATTTG TGTGGGGGAT CCAAAGAAGA CACAGACCGG TTTCAAGGGG
601      GACTCCGGGG GGCCCCTCGT GTGTAAGGAC GTAGCCCAAG GTATTCTCTC CTATGGAAAC
661      AAAAAAGGGA CACCTCCAGG AGTCTACATC AAGGTCTCAC ACTTCCTGCC CTGGATAAAG
721      AGAACAATGA AGCGCCTCTA A

 

Quality Control:cDNA sequencing results is available upon request.