Human GZMB Gene cDNA Clone / ORF Clone

GZMB cDNA Clone Datasheet

GZMB cDNA Clone Product Information

• Gene Bank Ref.ID: BC030195
• cDNA Size: 744
• Gene Synonym: HLP; CCPI; CGL1; CSPB; SECT; CGL-1; CSP-B; CTLA1; CTSGL1; GZMB
• Source: Human

GZMB cDNA Clone Description

• cDNA Description: ORF Clone of Homo sapiens granzyme B (granzyme 2, cytotoxic T-lymphocyte-associated serine esterase 1) DNA.
• Vector: Cloning Vector: pGEM-T Vector

  Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG

• Sequence Description: Identical with the Gene Bank Ref.ID sequence.

GZMB cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

GZMB cDNA Clone Usage Protocol

1. Cut along the circle edge of the spot (arrow points to) to get the filter paper.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to incubation at 95℃ for 15-30 min.
4. At the end of the incubation pulse vortex, or gently tap the tube.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

GZMB cDNA Clone Sequence and Quality Control

Sequence:

1        ATGCAACCAA TCCTGCTTCT GCTGGCCTTC CTCCTGCTGC CCAGGGCAGA TGCAGGGGAG
61       ATCATCGGGG GACATGAGGC CAAGCCCCAC TCCCGCCCCT ACATGGCTTA TCTTATGATC
121      TGGGATCAGA AGTCTCTGAA GAGGTGCGGT GGCTTCCTGA TACAAGACGA CTTCGTGCTG
181      ACAGCTGCTC ACTGTTGGGG AAGCTCCATA AATGTCACCT TGGGGGCCCA CAATATCAAA
241      GAACAGGAGC CGACCCAGCA GTTTATCCCT GTGAAAAGAC CCATCCCCCA TCCAGCCTAT
301      AATCCTAAGA ACTTCTCCAA CGACATCATG CTACTGCAGC TGGAGAGAAA GGCCAAGCGG
361      ACCAGAGCTG TGCAGCCCCT CAGGCTACCT AGCAACAAGG CCCAGGTGAA GCCAGGGCAG
421      ACATGCAGTG TGGCCGGCTG GGGGCAGACG GCCCCCCTGG GAAAACACTC ACACACACTA
481      CAAGAGGTGA AGATGACAGT GCAGGAAGAT CGAAAGTGCG AATCTGACTT ACGCCATTAT
541      TACGACAGTA CCATTGAGTT GTGCGTGGGG GACCCAGAGA TTAAAAAGAC TTCCTTTAAG
601      GGGGACTCTG GAGGCCCTCT TGTGTGTAAC AAGGTGGCCC AGGGCATTGT CTCCTATGGA
661      CGAAACAATG GCATGCCTCC ACGAGCCTGC ACCAAAGTCT CAAGCTTTGT ACACTGGATA
721      AAGAAAACCA TGAAACGCCA CTAA

 

Quality Control:cDNA sequencing results is available upon request.