Human GZMA Gene cDNA Clone / ORF Clone

Catalog	Vector (Price)	Quantity	In Stock	Operation
HG13325-G	Cloning vector ($95) pCMV / hygro ($295) pCMV / hygro - HA ($295) pCMV / hygro - His ($295) pCMV / hygro - Myc ($295) pCMV / hygro - FLAG ($295)		YES
* pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors. Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

GZMA cDNA Clone Datasheet

GZMA cDNA Clone Product Information

Gene Bank Ref.ID:	BC015739
cDNA Size:	789
Gene Synonym:	HFSP; CTLA3; GZMA
Source:	Human

GZMA cDNA Clone Description

cDNA Description:	ORF Clone of Homo sapiens granzyme A (granzyme 1, cytotoxic T-lymphocyte-associated serine esterase 3) DNA.
Vector:	Cloning Vector: pGEM-T Vector Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG
Sequence Description:	Identical with the Gene Bank Ref.ID sequence.

GZMA cDNA Clone Shipping

5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

GZMA cDNA Clone Usage Protocol

Cut along the circle edge of the spot (arrow points to) to get the filter paper.
Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
Transfer the tube to incubation at 95℃ for 15-30 min.
At the end of the incubation pulse vortex, or gently tap the tube.
Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

GZMA cDNA Clone Sequence and Quality Control

Sequence:

1        ATGAGGAACT CCTATAGATT TCTGGCATCC TCTCTCTCAG TTGTCGTTTC TCTCCTGCTA
61       ATTCCTGAAG ATGTCTGTGA AAAAATTATT GGAGGAAATG AAGTAACTCC TCATTCAAGA
121      CCCTACATGG TCCTACTTAG TCTTGACAGA AAAACCATCT GTGCTGGGGC TTTGATTGCA
181      AAAGACTGGG TGTTGACTGC AGCTCACTGT AACTTGAACA AAAGGTCCCA GGTCATTCTT
241      GGGGCTCACT CAATAACCAG GGAAGAGCCA ACAAAACAGA TAATGCTTGT TAAGAAAGAG
301      TTTCCCTATC CATGCTATGA CCCAGCCACA CGCGAAGGTG ACCTTAAACT TTTACAGCTG
361      ACGGAAAAAG CAAAAATTAA CAAATATGTG ACTATCCTTC ATCTACCTAA AAAGGGGGAT
421      GATGTGAAAC CAGGAACCAT GTGCCAAGTT GCAGGGTGGG GCAGGACTCA CAATAGTGCA
481      TCTTGGTCCG ATACTCTGAG AGAAGTCAAT ATCACCATCA TAGACAGAAA AGTCTGCAAT
541      GATCGAAATC ACTATAATTT TAACCCTGTG ATTGGAATGA ATATGGTTTG TGCTGGAAGC
601      CTCCGAGGTG GAAGAGACTC GTGCAATGGA GATTCTGGAA GCCCTTTGTT GTGCGAGGGT
661      GTTTTCCGAG GGGTCACTTC CTTTGGCCTT GAAAATAAAT GCGGAGACCC TCGTGGGCCT
721      GGTGTCTATA TTCTTCTCTC AAAGAAACAC CTCAACTGGA TAATTATGAC TATCAAGGGA
781      GCAGTTTAA

Quality Control:cDNA sequencing results is available upon request.