Rat GYPC Gene cDNA Clone / ORF Clone

Catalog	Vector (Price)	Quantity	In Stock	Operation
RG80373-G	Cloning vector ($95) pCMV / hygro ($295) pCMV / hygro - HA ($295) pCMV / hygro - His ($295) pCMV / hygro - Myc ($295) pCMV / hygro - FLAG ($295)		YES
* pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors. Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

GYPC cDNA Clone Datasheet

GYPC cDNA Clone Product Information

Gene Bank Ref.ID:	NM_001013233.1
cDNA Size:	288
Gene Synonym:	GPC; MGC124900; Gypc
Source:	Rat

GYPC cDNA Clone Description

cDNA Description:	ORF Clone of Rattus norvegicus glycophorin C (Gerbich blood group) DNA.
Vector:	Cloning Vector: pGEM-T Vector Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG
Sequence Description:	Identical with the Gene Bank Ref. ID sequence except for the point mutation 120 G/T not causing the amino acid variation.

GYPC cDNA Clone Shipping

5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

GYPC cDNA Clone Usage Protocol

Cut along the circle edge of the spot (arrow points to) to get the filter paper.
Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
Transfer the tube to incubation at 95℃ for 15-30 min.
At the end of the incubation pulse vortex, or gently tap the tube.
Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

GYPC cDNA Clone Sequence and Quality Control

Sequence:

1        ATGAGCAGCC CCGTCAGAAC GCCACCTCCT GAGCGCCTGG AGCCCAACCC AGGGATGTCC
61       TATGCCGTTA TGGAGATTGC CATCATTGCA GCTGTGATCA CTGCGGTGGC CTTGGTCCTT
121      GTCTGCCTCC TCTTTCTCAT GCTTCGCTAC CTGTACAGGC ACAAGGGCAC TTACTACACC
181      AATGAGGCCA AAGGCACGGA GTTTGCAGAA AGTGCGGATG CAGCTCTGCA AAGTGACCCT
241      GCCCTCCAGG ATGCCGGGGA CACCAGCAAG AAGGAATACT TTATCTGA

Quality Control:cDNA sequencing results is available upon request.