Human GRP Gene cDNA Clone / ORF Clone

GRP cDNA Clone Datasheet

GRP cDNA Clone Product Information

• Gene Bank Ref.ID: BC004488
• cDNA Size: 447
• Gene Synonym: BN; GRP-10; proGRP; preproGRP; GRP
• Source: Human

GRP cDNA Clone Description

• cDNA Description: ORF Clone of Homo sapiens gastrin-releasing peptide DNA.
• Vector: Cloning Vector: pGEM-T Vector

  Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG

• Sequence Description: Identical with the Gene Bank Ref.ID sequence.

GRP cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

GRP cDNA Clone Usage Protocol

1. Cut along the circle edge of the spot (arrow points to) to get the filter paper.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to incubation at 95℃ for 15-30 min.
4. At the end of the incubation pulse vortex, or gently tap the tube.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

GRP cDNA Clone Sequence and Quality Control

Sequence:

1        ATGCGCGGCC GTGAGCTCCC GCTGGTCCTG CTGGCGCTGG TCCTCTGCCT GGCGCCCCGG
61       GGGCGAGCGG TCCCGCTGCC TGCGGGCGGA GGGACCGTGC TGACCAAGAT GTACCCGCGC
121      GGCAACCACT GGGCGGTGGG GCACTTAATG GGGAAAAAGA GCACAGGGGA GTCTTCTTCT
181      GTTTCTGAGA GAGGGAGCCT GAAGCAGCAG CTGAGAGAGT ACATCAGGTG GGAAGAAGCT
241      GCAAGGAATT TGCTGGGTCT CATAGAAGCA AAGGAGAACA GAAACCACCA GCCACCTCAA
301      CCCAAGGCCC TGGGCAATCA GCAGCCTTCG TGGGATTCAG AGGATAGCAG CAACTTCAAA
361      GATGTAGGTT CAAAAGGCAA AGTTGGTAGA CTCTCTGCTC CAGGTTCTCA ACGTGAAGGA
421      AGGAACCCCC AGCTGAACCA GCAATGA

 

Quality Control:cDNA sequencing results is available upon request.