Mouse GRK5 Gene cDNA Clone / ORF Clone

GRK5 cDNA Clone Datasheet

GRK5 cDNA Clone Product Information

• Gene Bank Ref.ID: NM_018869.3
• cDNA Size: 1773
• Gene Synonym: Gprk5; Grk5
• Source: Mouse

GRK5 cDNA Clone Description

• cDNA Description: ORF Clone of Mus musculus G protein-coupled receptor kinase 5 DNA.
• Vector: pGEM-T Vector
• Sequence Description: Identical with the Gene Bank Ref.ID sequence except for three point mutations: 1221 G/A , 1338 T/C , 1671 T/G not causing the amino acid variation.

GRK5 cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

GRK5 cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95℃ for 15-30 min.
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

GRK5 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGAGCTGG AAAACATCGT GGCCAACACG GTCTTGCTGA AAGCCCGGGA AGGGGGTGGA
61       GGAAAGCGCA AAGGGAAAAG CAAGAAGTGG AAGGAAATCC TGAAGTTTCC TCACATCAGC
121      CAGTGTGAAG ACCTCCGAAG GACCATAGAC AGAGATTACT ACAGTCTATG TGACAAGCAA
181      CCAATTGGGA GACTGCTTTT TCGACAGTTC TGTGAAACCA GGCCTGGGCT GGAGTGCTAC
241      ATTCAGTTCC TGGACTTAGT GGCAGAATAT GAAATTACTC CAGATGAAAA CCTTGGGGCG
301      AAGGGGAAGG AAATAATGAC CAAGTACCTC ACTCCAAAGT CCCCAGTCTT CATTGCCCAA
361      GTTGGACAGG ACCTGGTCTC CCAGACAGAG AAGAAGCTCC TGCAGAGCCC CTGCAAAGAA
421      CTCTTCTCTG CTTGTGCTCA GTCTGTCCAT GACTACTTGA AGGGAGACCC CTTCCACGAG
481      TACCTGGATA GCATGTATTT TGACCGTTTT CTGCAGTGGA AATGGTTAGA AAGACAACCA
541      GTGACCAAAA ACACTTTCCG GCAGTACCGA GTGCTGGGCA AAGGGGGCTT TGGAGAGGTC
601      TGTGCCTGCC AGGTTCGGGC CACTGGTAAA ATGTATGCTT GTAAACGCTT AGAGAAGAAG
661      AGGATCAAAA AGAGGAAAGG CGAATCCATG GCACTCAACG AAAAGCAGAT TCTTGAGAAG
721      GTCAACAGCC AGTTTGTGGT CAACCTGGCC TATGCCTATG AAACCAAAGA TGCACTATGC
781      CTGGTTCTGA CCATTATGAA TGGTGGTGAC CTGAAGTTTC ACATCTACAA TATGGGGAAT
841      CCTGGCTTTG AGGAAGAGCG AGCCTTATTT TATGCAGCTG AGATCCTCTG TGGCCTAGAA
901      GACTTACACC GTGAGAACAC TGTCTATAGA GATCTAAAAC CCGAAAACAT CTTGCTGGAT
961      GATTATGGCC ACATAAGGAT CTCAGACCTC GGACTGGCCG TGAAGATCCC CGAGGGAGAC
1021     CTTATCCGTG GCCGGGTAGG CACTGTTGGC TACATGGCCC CAGAAGTTCT GAACAACCAG
1081     CGATATGGAC TGAGCCCTGA CTACTGGGGC CTGGGCTGCC TCATCTATGA GATGATTGAA
1141     GGCCAGTCAC CATTTCGAGG TCGCAAGGAG AAGGTTAAGC GGGAAGAGGT GGATCGCCGG
1201     GTGCTGGAGA CTGAGGAAGT ATATTCCTCC AAGTTCTCTG AAGAGGCCAA GTCCATCTGC
1261     AACATGCTGC TCACCAAAGA CTCGAAGCAG AGGCTGGGCT GCCAGGAGGA GGGGGCCGCC
1321     GAGGTCAAGA GGCACCCGTT CTTCAGGAAC ATGAACTTTA AGCGCCTGGA GGCTGGGATG
1381     TTGGACCCTC CCTTCGTTCC AGATCCCCGG GCTGTATACT GCAAGGATGT GCTGGACATT
1441     GAGCAGTTCT CCACTGTGAA AGGTGTCAAC CTGGACCATA CGGACGATGA TTTTTACTCA
1501     AAGTTCTCTA CAGGCTCTGT GCCAATTCCA TGGCAAAATG AGATGATAGA AACAGAATGT
1561     TTCAAGGAGC TGAATGTGTT CGGACCTAAC GGTACCCTCT CACCAGACCT GAACAGAAGT
1621     CAGCCTCCAG AACCGCCAAA GAAAGGGCTG TTCCACAGAC TCTTCAGGCG GCAGCATCAA
1681     AGCAATTCCA AGAGTTCACC TACTCCTAAG ACCAGTTGTA ACCACCGAAT AAATTCAAAC
1741     CACATCAATT CAAACTCCAC TGGAAGCAGC TAG

 

Quality Control:cDNA sequencing results is available upon request.