GRK5 Antibody (Antigen Affinity Purified)

GRK5 Antibody ( Antigen Affinity Purified ) Datasheet

GRK5 Antibody Product Information

• Immunogen: Recombinant Human GRK5 protein (Catalog#10839-H08B)
• Antibody Type: Rabbit Polyclonal Antibody ( Antibody Purification Platform )
• Ig Type: Rabbit IgG
• Formulation: 0.2 μm filtered solution in PBS with 5% trehalose
• Preparation: Produced in rabbits immunized with purified, recombinant Human GRK5 (rh GRK5; Catalog#10839-H08B; NP_005299.1; Met 1-Ser 590). GRK5 specific IgG was purified by Human GRK5 affinity chromatography.

GRK5 Antibody Usage Guide

• Specificity: Human GRK5
• Western blot: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human GRK5 in WB. Using a DAB detection system, the detection limit for Human GRK5 is approximately 4 ng/lane under non-reducing conditions and 1 ng/lane under reducing conditions.
• Direct ELISA: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human GRK5. The detection limit for Human GRK5 is approximately 0.039 ng/well.
• Storage: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
  Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

GRK5 Antibody Background

G protein-coupled receptor kinase 5, also known as G protein-coupled receptor kinase GRK5 and GRK5, is a member of the protein kinase superfamily, AGC Ser/Thr protein kinase family and GPRK subfamily. GRKs specifically phosphorylate agonist-occupied G protein-coupled receptors at the inner surface of the plasma membrane (PM), leading to receptor desensitization. GRKs utilize a variety of mechanisms to bind tightly, and sometimes reversibly, to cellular membranes. GRKs play an important role in mediating agonist-specific desensitization of numerous G protein-coupled receptors. GRK5 contains oneAGC-kinase C-terminal domain, oneprotein kinase domain and oneRGS domain. GRK5 specifically phosphorylates the activated forms of G protein-coupled receptors. Phospholipid-stimulated autophosphorylation may represent a novel mechanism for membrane association and regulation of GRK5 activity. GRK5 deficiency significantly exaggerates microgliosis and astrogliosis in the presence of an inflammatory initiator, such as the excess fibrillar Abeta and the subsequent active inflammatory reactions. GRK5 deficiency has been linked to early Alzheimer's disease in humans and mouse models of the disease.

References

1. Kunapuli P. et al., 1994, J Biol Chem. 269 (14): 10209-12.
2. Millman EE. et al., 2004, Br J Pharmacol. 141 (2): 277-84.
3. Thiyagarajan MM. et al., 2004, J Biol Chem. 279 (17): 17989-95.
4. Suo Z. et al., 2007, Neurobiol Aging. 28 (12): 1873-88.
5. Li L. et al., 2008, J Neuroinflammation. 5: 24.