Human GRK1 Gene cDNA Clone / ORF Clone

Human GRK1 cDNA Clone Datasheet

GRK1 cDNA Clone Product Information

• Gene Bank Ref.ID: NM_002929.2
• cDNA Size: 1692
• Gene Synonym: RK; RHOK; GPRK1; GRK1
• Source: Human

GRK1 cDNA Clone Description

• cDNA Description: ORF Clone of Homo sapiens G protein-coupled receptor kinase 1 DNA.
• Vector: pGEM-T Vector

• Sequence Description: Identical with the Gene Bank Ref.ID sequence.

GRK1 cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

GRK1 cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95℃ for 15-30 min.
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

GRK1 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGATTTCG GGTCTTTGGA GACCGTGGTG GCCAACTCTG CCTTCATCGC CGCCCGAGGC
61       AGCTTTGACG GCAGCAGCTC CCAACCCTCC CGGGACAAGA AGTACCTGGC CAAGCTCAAG
121      CTGCCCCCGC TGTCCAAGTG TGAGTCCCTC CGCGACAGCC TCAGCCTGGA GTTTGAGAGT
181      GTGTGCTTGG AGCAGCCCAT CGGCAAGAAG CTCTTTCAGC AGTTCCTACA ATCGGCAGAG
241      AAGCACCTGC CGGCCCTGGA GCTCTGGAAA GACATCGAGG ACTATGACAC GGCAGACAAT
301      GACCTCCAGC CACAGAAGGC CCAGACCATC CTGGCCCAGT ACCTGGACCC CCAGGCCAAA
361      CTCTTCTGCA GCTTCCTGGA TGAGGGGATA GTGGCGAAGT TTAAGGAGGG GCCTGTGGAG
421      ATCCAGGACG GGCTCTTCCA GCCCCTGCTG CAGGCCACCC TGGCACACCT GGGCCAAGCC
481      CCCTTCCAGG AGTACCTGGG CAGCCTGTAC TTCCTGAGGT TCCTGCAGTG GAAGTGGCTG
541      GAAGCCCAGC CCATGGGGGA GGACTGGTTC CTGGACTTCA GGGTCCTGGG GAAAGGGGGC
601      TTCGGGGAGG TGTCGGCCTG CCAGATGAAG GCGACCGGCA AGCTGTATGC CTGCAAGAAG
661      CTGAACAAGA AGCGGCTGAA GAAGAGGAAG GGCTACCAGG GTGCTATGGT GGAGAAGAAG
721      ATTCTGATGA AAGTACACAG CAGGTTCATC GTGTCTCTGG CCTATGCGTT TGAAACCAAA
781      GCCGACCTCT GTCTGGTGAT GACCATCATG AACGGAGGTG ACATCAGGTA CCACATCTAC
841      AACGTGAATG AGGAGAACCC TGGCTTCCCG GAGCCGCGCG CCCTCTTCTA CACGGCGCAG
901      ATCATCTGCG GCCTGGAGCA CCTGCACCAG AGGCGGATCG TCTACCGCGA CCTCAAGCCC
961      GAGAACGTGC TGCTGGACAA TGACGGCAAT GTCCGGATCT CTGACCTTGG GCTGGCCGTG
1021     GAGCTGCTGG ACGGACAGAG CAAGACCAAG GGCTACGCAG GGACCCCAGG TTTCATGGCC
1081     CCCGAGCTCC TGCAGGGCGA GGAGTACGAC TTCTCCGTGG ACTACTTTGC CCTGGGGGTC
1141     ACCCTGTATG AGATGATTGC GGCCAGAGGA CCCTTCCGAG CCCGTGGAGA GAAGGTGGAG
1201     AACAAGGAGC TGAAGCACCG GATCATCTCA GAGCCCGTGA AGTACCCTGA TAAGTTCAGC
1261     CAGGCCAGCA AGGACTTCTG CGAGGCGCTG CTGGAGAAGG ACCCGGAGAA GCGCCTGGGG
1321     TTCAGAGATG AGACCTGCGA CAAGCTCCGT GCCCACCCCC TCTTCAAGGA CCTTAACTGG
1381     AGGCAGCTGG AGGCTGGGAT GCTGATGCCC CCTTTCATCC CAGACTCCAA AACTGTCTAC
1441     GCAAAGGATA TTCAGGACGT GGGTGCCTTT TCCACCGTCA AAGGTGTGGC CTTTGACAAA
1501     ACAGACACAG AATTCTTTCA GGAATTTGCC ACTGGCAACT GCCCCATCCC CTGGCAGGAG
1561     GAGATGATCG AGACGGGCAT CTTTGGCGAG CTGAACGTGT GGCGCTCGGA CGGTCAGATG
1621     CCGGACGACA TGAAGGGCAT CTCCGGGGGC TCCAGCTCCT CGTCCAAGTC AGGGATGTGT
1681     CTGGTTTCCT AG

 

Quality Control:cDNA sequencing results is available upon request.