Anti-GPT2 Antibody

Cat: 80002-RP02
Contact us:
+86-400-890-9989
order@sinobiological.com
Distributors
All GPT2 Reagents
CRO Services
Product recommended

All GPT2 Reagents
Anti-GPT2 Antibody (Rabbit Polyclonal antibody) General Information
Product name
Anti-GPT2 Antibody
Validated applications
ELISA,IHC-P
Species reactivity
Reacts with: Rat
Specificity
Rat GPT2
Immunogen
Recombinant Rat GPT2 protein (Catalog#80002-R08B)
Preparation
Produced in rabbits immunized with purified, recombinant Rat GPT2 (rR GPT2; Catalog#80002-R08B; Met 1-Ser 522; NP_001012057.1). GPT2 specific IgG was purified by Rat GPT2 affinity chromatography.
Source
Polyclonal Rabbit IgG
Purification
Protein A & Antigen Affinity
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Anti-GPT2 Antibody (Rabbit Polyclonal antibody) Validated Applications
Application Dilution Notes
ELISA 1:1000-1:2000  
IHC-P 1:500-1:2000  

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-GPT2 Antibody (Rabbit Polyclonal antibody) Images
Rat GPT2 Immunohistochemistry(IHC) 3122
Immunochemical staining of rat GPT2 in rat liver with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).
Rat GPT2 Immunohistochemistry(IHC) 3123
Immunochemical staining of rat GPT2 in rat brain with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections). The left panel: tissue incubated with primary antibody; The right panel: tissue incubated with the mixture of primary antibody and antigen (recombinant protein).
GPT2 Background Information

Alanine aminotransferase (ALT), also known as glutamate pyruvate transaminase (Gpt), is a pyridoxal enzyme which catalyses the reversible interconversion of L-alanine and 2-oxoglutalate to pyruvate and L-glutamate, and plays a key role in the intermediary metabolism of glucose and amino acids. As a key enzyme for gluconeogenesis, Gpt is a widely-used serum marker for liver injury. Two ALT isoenzymes have been identified, ALT1 and ALT2 (GPT1 and GPT2), which are encoded by separate genes and share significant sequence homology, but differ in their expression patterns. Gpt1 is widely distributed and mainly expressed in intestine, liver, fat tissues, colon, muscle, and heart, in the order of high to low expression level, whereas Gpt2 expression is more restricted, mainly in liver, muscle, brain, and white adipose tissue. It has been reported that hepatic ALT2 protein is approximately four times higher in male rats than in female rats.

Full Name
glutamic pyruvate transaminase (alanine aminotransferase) 2
References
  • Matthews, C.C. et al., 2002, Genomics. 79: 445-450.
  • Jadhao, S.B. et al., 2004, Hepatology. 39: 1297-1302.
  • Schindhelm, R.K. et al., 2005, Eur. J.Clin. Invest. 35:369-374.
  • Lindblom, P. et al., 2007, Arch Biochem Biophys. 466 (1): 66-77.
  • Liu, L.et al., 2008, Protein Expr Purif. 60 (2): 225-231.
  • Yang, R. Z. et al., 2009, Hepatology. 49 (2): 598-607.
  • Product Description Host Clonality Application Catalog# (PDF)
    Anti-GPT2 Antibody Rabbit Monoclonal ELISA 80002-R033
    Anti-GPT2 Antibody Rabbit Polyclonal ELISA 80002-RP01
    info info
    添加购物车成功! 添加购物车失败!