GPT2 Antibody (Antigen Affinity Purified)

GPT2 Antibody (Antigen Affinity Purified) Datasheet

GPT2 Antibody Product Information

• Immunogen: Recombinant Rat GPT2 protein (Catalog #80002-R08B)
• Antibody Type: Rabbit Polyclonal Antibody ( Antibody Purification Platform )
• Ig Type: Rabbit IgG
• Formulation: 0.2 μm filtered solution in PBS with 5% trehalose
• Preparation: Produced in rabbits immunized with purified, human cell-derived, recombinant Rat GPT2 (rR GPT2; Catalog #80002-R08B; Met 1-Ser 522; NP_001012057.1). GPT2 specific IgG was purified by Rat GPT2 affinity chromatography.

GPT2 Antibody Usage Guide

• Specificity: Rat GPT2
• Western blot: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Rat Gpt2 in WB. Using a DAB detection system, the detection limit for Rat Gpt2 is approximately 4 ng/lane under non-reducing conditions and 2 ng/lane under reducing conditions.
• Direct ELISA: This antibody can be used at 0.5-1 μg/mL with the appropriate secondary reagents to detect Rat Gpt2. The detection limit for Rat Gpt2 is approximately 0.039 ng/well.
• Storage: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
  Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

GPT2 Antibody Background

Alanine aminotransferase (ALT), also known as glutamate pyruvate transaminase (Gpt), is a pyridoxal enzyme which catalyses the reversible interconversion of L-alanine and 2-oxoglutalate to pyruvate and L-glutamate, and plays a key role in the intermediary metabolism of glucose and amino acids. As a key enzyme for gluconeogenesis, Gpt is a widely-used serum marker for liver injury. Two ALT isoenzymes have been identified, ALT1 and ALT2 (GPT1 and GPT2), which are encoded by separate genes and share significant sequence homology, but differ in their expression patterns. Gpt1 is widely distributed and mainly expressed in intestine, liver, fat tissues, colon, muscle, and heart, in the order of high to low expression level, whereas Gpt2 expression is more restricted, mainly in liver, muscle, brain, and white adipose tissue. It has been reported that hepatic ALT2 protein is approximately four times higher in male rats than in female rats.

References

1. Matthews, C.C. et al., 2002, Genomics. 79: 445-450.
2. Jadhao, S.B. et al., 2004, Hepatology. 39: 1297-1302.
3. Schindhelm, R.K. et al., 2005, Eur. J.Clin. Invest. 35:369-374.
4. Lindblom, P. et al., 2007, Arch Biochem Biophys. 466 (1): 66-77.
5. Liu, L.et al., 2008, Protein Expr Purif. 60 (2): 225-231.
6. Yang, R. Z. et al., 2009, Hepatology. 49 (2): 598-607.